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Literature summary for 3.2.1.4 extracted from

  • Mangan, D.; Cornaggia, C.; McKie, V.; Kargelis, T.; McCleary, B.V.
    A novel automatable enzyme-coupled colorimetric assay for endo-1,4-beta-glucanase (cellulase) (2016), Anal. Bioanal. Chem., 408, 4159-4168 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis rapid, selective, quantitative assay based on substrate 44-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside and reaction of product 4,6-O-(3-oxobutylidene)-beta-D-cellotriose with beta-glucosidase Trichoderma longibrachiatum
analysis rapid, selective, quantitative assay based on substrate 44-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside and reaction of product 4,6-O-(3-oxobutylidene)-beta-D-cellotriose with beta-glucosidase Bacillus amyloliquefaciens
analysis rapid, selective, quantitative assay based on substrate 44-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside and reaction of product 4,6-O-(3-oxobutylidene)-beta-D-cellotriose with beta-glucosidase Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger O74705
-
-
Bacillus amyloliquefaciens Q8RPQ6
-
-
Trichoderma longibrachiatum Q12714
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Trichoderma longibrachiatum
-
commercial preparation
-
Bacillus amyloliquefaciens
-
commercial preparation
-
Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O
-
Trichoderma longibrachiatum 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside
-
?
4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O
-
Bacillus amyloliquefaciens 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside
-
?
4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O
-
Aspergillus niger 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside
-
?

Synonyms

Synonyms Comment Organism
Egl1
-
Trichoderma longibrachiatum
EglA
-
Aspergillus niger
EngA
-
Bacillus amyloliquefaciens