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Literature summary for 3.2.1.4 extracted from

  • Li, D.; Lu, M.; Li, Y.; Lu, J.
    Purification and characterization of an endocellulase from the thermophilic fungus Chaetomium thermophilum CT2 (2003), Enzyme Microb. Technol., 33, 932-937 .
No PubMed abstract available
Search for more literature for 3.2.1.4

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 1% residual activity Thermochaetoides thermophila
Ba2+ 1 mM, 38% residual activity Thermochaetoides thermophila
Cu2+ 1 mM, 10% residual activity Thermochaetoides thermophila
Fe2+ 1 mM, no residual activity Thermochaetoides thermophila
Hg2+ 1 mM, 2% residual activity Thermochaetoides thermophila
Mg2+ 10 mM, 12% residual activity Thermochaetoides thermophila
Mn2+ 1 mM, 46% residual activity Thermochaetoides thermophila
NH4+ 10 mM, 9% residual activity Thermochaetoides thermophila
Zn2+ 1 mM, 19% residual activity Thermochaetoides thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethyl cellulose Km value 4.6 mg/ml, pH 5.0, 60°C Thermochaetoides thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermochaetoides thermophila
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 10 mM, 110% of initial activity Thermochaetoides thermophila
Na+ 10 mM, 119% of initial activity Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69800
-
 gel filtration Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
-
-
Thermochaetoides thermophila CT2
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content 18.9% Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.7
-
 pH 5.0, 60°C Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Thermochaetoides thermophila ?
-
 ?
avicel + H2O
-
 Thermochaetoides thermophila CT2 ?
-
 ?
carboxymethyl cellulose + H2O best substrate tested Thermochaetoides thermophila ?
-
 ?
carboxymethyl cellulose + H2O best substrate tested Thermochaetoides thermophila CT2 ?
-
 ?
filter paper + H2O
-
 Thermochaetoides thermophila ?
-
 ?
filter paper + H2O
-
 Thermochaetoides thermophila CT2 ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Thermochaetoides thermophila ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Thermochaetoides thermophila CT2 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 67800, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, stable Thermochaetoides thermophila
70
-
 half-life 45 min Thermochaetoides thermophila
80
-
 half-life 24 min Thermochaetoides thermophila
90
-
 half-life 7 min Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Thermochaetoides thermophila