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Literature summary for 3.2.1.4 extracted from
- Properties of enzyme preparations and homogeneous enzymes - endoglucanases EG2 Penicillium verruculosum and LAM Myceliophthora thermophila (2015), Biochemistry (Moscow), 80, 473-482 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | oligosaccharides with degree of polymerization 2-10 are formed by hydrolysis of beta-glucan. The recombinant enzyme preparations are fast and effective in decreasing the reduced viscosity of wholegrain barley extract than some commercial enzyme preparations | Talaromyces verruculosus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Talaromyces verruculosus | Talaromyces verruculosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
beta-Glucan | Km value 22.8 mg/ml, pH 5.0, 50°C | Talaromyces verruculosus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces verruculosus | A0A1U7Q1U3 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 75 | - |
substrate beta-glucan, pH 5.0, 50°C | Talaromyces verruculosus |
| 76 | - |
substrate carboxymethyl cellulose, pH 5.0, 50°C | Talaromyces verruculosus |
| 94 | - |
substrate lichenan, pH 5.0, 50°C | Talaromyces verruculosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | degree of hydrolysis reaches 10%, products are oligosaccharides with degree of polymerization 2-10 | Talaromyces verruculosus | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Talaromyces verruculosus | ? | - |
? | |
| lichenan + H2O | - |
Talaromyces verruculosus | ? | - |
? | |
| additional information | no substrates: laminarin, curdlan | Talaromyces verruculosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Talaromyces verruculosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EG2 | - |
Talaromyces verruculosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 61 | 80 | - |
Talaromyces verruculosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | 85 | more than 50% of maximum activity within | Talaromyces verruculosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 156 | - |
beta-Glucan | pH 5.0, 50°C | Talaromyces verruculosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.9 | 4.9 | - |
Talaromyces verruculosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.4 | 5.6 | more than 50% of maximum activity within | Talaromyces verruculosus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
beta-Glucan | kcat/Km value 6.6 ml/s/mg, pH 5.0, 50°C | Talaromyces verruculosus | |
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