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Literature summary for 3.2.1.4 extracted from
- Stability of endoglucanases from mesophilic fungus and thermophilic bacterium in acidified polyols (2014), Enzyme Microb. Technol., 61-62, 55-60.
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethylene glycol | 95.6% glycerol, plus 0.1% H2SO4, 36% residual activity | Thermotoga maritima |
| Ethylene glycol | 95.6%, plus 0.1% H2SO4, 36% residual activity | Trichoderma longibrachiatum |
| Glycerol | 96%, plus 0.1% H2SO4, 35% residual activity | Thermotoga maritima |
| Glycerol | 96%, plus 0.1% H2SO4, 80% residual activity | Trichoderma longibrachiatum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
| Trichoderma longibrachiatum | Q12714 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Thermotoga maritima | - |
| commercial preparation | - |
Trichoderma longibrachiatum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 127.2 | - |
presence of 96% glycerol, pH 4.8, 4°C | Thermotoga maritima |
| 139.6 | - |
pH 4.8, 4°C | Thermotoga maritima |
| 264.7 | - |
presence of 96% glycerol, pH 4.8, 4°C | Trichoderma longibrachiatum |
| 331.9 | - |
pH 4.8, 4°C | Trichoderma longibrachiatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Thermotoga maritima | ? | - |
? |  |
| carboxymethyl cellulose + H2O | - |
Trichoderma longibrachiatum | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
presence of 96%, plus 0.1% H2SO4, half-life 4.5 min | Thermotoga maritima |
| 80 | - |
presence of 96%glycerol, plus 0.1% H2SO4, half-life 4.5 min | Trichoderma longibrachiatum |
| 90 | - |
5 min, 87% residiual activity | Thermotoga maritima |
| 90 | - |
5 min, 87% residiual activity | Trichoderma longibrachiatum |
| 100 | - |
5 min, 5% residual activity | Thermotoga maritima |
| 100 | - |
5 min, 75% residual activity | Trichoderma longibrachiatum |
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