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Literature summary for 3.2.1.4 extracted from

  • Kim, S.C.; Kang, S.H.; Choi, E.Y.; Hong, Y.H.; Bok, J.D.; Kim, J.Y.; Lee, S.S.; Choi, Y.J.; Choi, I.S.; Cho, K.K.
    Cloning and characterization of an endoglucanase gene from Actinomyces sp. Korean Native Goat 40 (2016), Asian-Australas. J. Anim. Sci., 29, 126-133.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Actinomyces sp. 40

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51400
-
 x * 51400 and x * 57100, without and with signal peptide, respectively, SDS-PAGE Actinomyces sp. 40
57100
-
 x * 51400 and x * 57100, without and with signal peptide, respectively, SDS-PAGE Actinomyces sp. 40

Organism

Organism UniProt Comment Textmining
Actinomyces sp. 40 O66064
-
-
Actinomyces sp. 40 Korean Native Goat 40 O66064
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains signal peptide-like amino acids Actinomyces sp. 40

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Actinomyces sp. 40

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Actinomyces sp. 40 ? 8.1% of the activity with carboxymethyl cellulose ?
avicel + H2O
-
 Actinomyces sp. 40 Korean Native Goat 40 ? 8.1% of the activity with carboxymethyl cellulose ?
carboxymethyl cellulose + H2O
-
 Actinomyces sp. 40 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Actinomyces sp. 40 Korean Native Goat 40 ?
-
 ?
cellobiose + H2O
-
 Actinomyces sp. 40 2 D-glucose 26% of the activity with carboxymethyl cellulose ?
cellobiose + H2O
-
 Actinomyces sp. 40 Korean Native Goat 40 2 D-glucose 26% of the activity with carboxymethyl cellulose ?
xylan + H2O
-
 Actinomyces sp. 40 ? 6.1% of the activity with carboxymethyl cellulose ?
xylan + H2O
-
 Actinomyces sp. 40 Korean Native Goat 40 ? 6.1% of the activity with carboxymethyl cellulose ?

Subunits

Subunits Comment Organism
? x * 51400 and x * 57100, without and with signal peptide, respectively, SDS-PAGE Actinomyces sp. 40

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Actinomyces sp. 40

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 more than 90% of maximum activity Actinomyces sp. 40

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Actinomyces sp. 40