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Literature summary for 3.2.1.4 extracted from
- Fusion of cellulose binding domain from Trichoderma reesei CBHI to Cryptococcus sp. S-2 cellulase enhances its binding affinity and its cellulolytic activity to insoluble cellulosic substrates (2013), Enzyme Microb. Technol., 52, 241-246.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Trichoderma reesei |
- |
Cryptococcus sp. (in: Fungi) |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | fusion of the cellulose binding domain of cellobiohydrolase I from Trichoderma reesei to the C-terminus of Cryptococcus sp. carboxymethyl cellulase and expression in a recombinant expression system of Cryptococcus sp. S-2. The recombinant fusion enzymes display optimal pH similar to those of the native enzyme. Compared with Cryptococcus sp. carboxymethyl cellulase, the recombinant fusion enzymes have acquired an increased binding affinity to insoluble cellulose, and the cellulolytic activity toward insoluble cellulosic substrates, SIGMACELL and Avicel, is higher than that of native enzyme, confirming the presence of cellulose binding domains improve the binding and the cellulolytic activity of carboxymethyl cellulase on insoluble substrates | Trichoderma reesei |
| additional information | fusion of the cellulose binding domain of cellobiohydrolase I from Trichoderma reesei to the C-terminus of Cryptococcus sp. carboxymethyl cellulase and expression in a recombinant expression system of Cryptococcus sp. S-2. The recombinant fusion enzymes display optimal pH similar to those of the native enzyme. Compared with Cryptococcus sp. carboxymethyl cellulase, the recombinant fusion enzymes have acquired an increased binding affinity to insoluble cellulose, and the cellulolytic activity toward insoluble cellulosic substrates, SIGMACELL and Avicel, is higher than that of native enzyme, confirming the presence of cellulose binding domains improve the binding and the cellulolytic activity of carboxymethyl cellulase on insoluble substrates | Cryptococcus sp. (in: Fungi) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cryptococcus sp. (in: Fungi) | - |
- |
- |
| Trichoderma reesei | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBHI | - |
Trichoderma reesei |
| cellobiohydrolase | - |
Trichoderma reesei |
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