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Literature summary for 3.2.1.4 extracted from
- Purification and characterization of cellulase from North Pacific krill (Euphausia pacifica). Analysis of cleavage specificity of the enzyme (2012), Comp. Biochem. Physiol. B, 163, 324-333.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
gel filtration | Euphausia pacifica |
| 48000 | - |
1 * 48000, SDS-PAGE | Euphausia pacifica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euphausia pacifica | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | total hexose content is 0.044 mg/mg protein | Euphausia pacifica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Euphausia pacifica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42.3 | - |
substrate carboxymethyl cellulose, pH 5.5, 40°C | Euphausia pacifica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | enzyme hydrolyzes internal beta-1,4 glycosidic bonds within lichenan as well as carboxymethyl cellulose to release oligosaccharides and glucose | Euphausia pacifica | cellotriose + cellobiose + D-glucose | major products | ? |  |
| cellohexaose + H2O | - |
Euphausia pacifica | cellotriose + cellobiose + D-glucose | - |
? | |
| cellopentaose + H2O | - |
Euphausia pacifica | cellotriose + cellobiose + D-glucose | - |
? | |
| cellotetraose + H2O | - |
Euphausia pacifica | cellotriose + cellobiose + D-glucose | - |
? | |
| filter paper + H2O | poor substrate | Euphausia pacifica | cellotriose + cellobiose + D-glucose | major products | ? | |
| lichenan + H2O | enzyme hydrolyzes internal beta-1,4 glycosidic bonds within lichenan as well as carboxymethyl cellulose to release oligosaccharides and glucose | Euphausia pacifica | cellotriose + cellobiose + D-glucose | major products | ? | |
| additional information | no substrates: cellotriose, cellobiose | Euphausia pacifica | ? | - |
? | |
| sea lettuce + H2O | - |
Euphausia pacifica | cellotriose + cellobiose + D-glucose | major products | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 48000, SDS-PAGE | Euphausia pacifica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Euphausia pacifica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Euphausia pacifica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 4°C, 4 h, retains more than 70% of maximum activity | Euphausia pacifica |
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