Crystallization (Comment) | Organism |
---|---|
mutants Y16G, Y61GG and Y61del in complex with substrate beta-glucan. Mutations at the Tyr61 site do not affect the overall protein structure, but local perturbations might diminish the substrate-binding strength | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
R60A | about 45% of wild-type activity | Thermotoga maritima |
R60K | about 70% of wild-type activity | Thermotoga maritima |
Y61A | about 120% of wild-type activity | Thermotoga maritima |
Y61del | about 10% of wild-type activity | Thermotoga maritima |
Y61F | about 140% of wild-type activity | Thermotoga maritima |
Y61G | about 170% of wild-type activity. Mutant also shows a wider range of working temperatures than does the wild type, along with retention of the hyperthermostability. The kcat and Km values of Y61G are both higher than those of the wild type. The higher endoglucanase activity is probably due to facile dissociation of the cleaved sugar moiety at the reducing end | Thermotoga maritima |
Y61GG | insertion mutant, about 40% of wild-type activity | Thermotoga maritima |
Y61R | about 70% of wild-type activity | Thermotoga maritima |
Y61W | about 80% of wild-type activity | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
beta-Glucan | Km value for wild-type is 2.08 mg/ml, for mutant Y61G 3.75 mg/ml, pH 5.0, 85°C | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q60032 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | - |
Thermotoga maritima | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CelA | - |
Thermotoga maritima |
cellulase 12A | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
wild-type | Thermotoga maritima |
100 | - |
mutant Y61G | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life 8 h, both wild-type and mutant Y61G | Thermotoga maritima |
95 | - |
half-life 40 min, both wild-type and mutant Y61G | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
791 | - |
beta-Glucan | wild-type, pH 5.0, 85°C | Thermotoga maritima | |
1155 | - |
beta-Glucan | mutant Y61G, pH 5.0, 85°C | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
beta-Glucan | Kcat/Km value for wild-type is 402 mg per s and ml, for mutant Y61G 313 mg per s and ml, pH 5.0, 85°C | Thermotoga maritima |