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Literature summary for 3.2.1.4 extracted from

  • Kupski, L.; Pagnussatt, F.A.; Buffon, J.G.; Furlong, E.B.
    Endoglucanase and total cellulase from newly isolated Rhizopus oryzae and Trichoderma reesei: production, characterization, and thermal stability (2014), Appl. Biochem. Biotechnol., 172, 458-468.
    View publication on PubMed
Search for more literature for 3.2.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethyl cellulose KM value is 0.01 mg/ml, pH 4.8, 50°C Rhizopus arrhizus
additional information
-
 carboxymethyl cellulose KM value is 0.20 mg/ml according to Lineweaver-Burk method and 0.28 mg/ml according to Hanes-Wolf method, pH 4.8, 50°C Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
-
-
Rhizopus arrhizus CCT 7560
-
-
-
Trichoderma reesei
-
-
-
Trichoderma reesei QM 9414
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Rhizopus arrhizus ?
-
 ?
carboxymethyl cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
carboxymethyl cellulose + H2O
-
 Rhizopus arrhizus CCT 7560 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Trichoderma reesei QM 9414 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Rhizopus arrhizus
60
-
-
 Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life 7.2 h Trichoderma reesei
80
-
 half-life 1.9 h Trichoderma reesei
80
-
 half-life 77 h Rhizopus arrhizus
100
-
 half-life 43.3 h Rhizopus arrhizus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Rhizopus arrhizus
6
-
-
 Trichoderma reesei