Any feedback?
Literature summary for 3.2.1.4 extracted from
- Purification and biochemical characterization of a novel thermo-stable carboxymethyl cellulase from Azorean isolate Bacillus mycoides S122C (2012), Appl. Biochem. Biotechnol., 168, 2191-2204.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 10 mM, 51% residual activity | Bacillus mycoides |  |
| CaCl2 | 5 mM, 52% residual activity | Bacillus mycoides | |
| CuSO4 | 5 mM, 38% residual activity | Bacillus mycoides | |
| dithiothreitol | 10 mM, 67% residual activity | Bacillus mycoides | |
| EDTA | 10 mM, 28% residual activity | Bacillus mycoides | |
| FeCl2 | 5 mM, 38% residual activity | Bacillus mycoides | |
| HgCl2 | 5 mM, 25% residual activity | Bacillus mycoides | |
| KCl | 5 mM, 74% residual activity | Bacillus mycoides | |
| MgCl2 | 5 mM, 52% residual activity | Bacillus mycoides | |
| NiCl2 | 5 mM, 48% residual activity | Bacillus mycoides | |
| sodium dodecylsulfate | 1%, 73% residual activity | Bacillus mycoides | |
| Tween-20 | 1%, 79% residual activity | Bacillus mycoides | |
| Urea | 10 mM, 45% residual activity | Bacillus mycoides | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus mycoides | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, SDS-PAGE | Bacillus mycoides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus mycoides | - |
- |
- |
| Bacillus mycoides S122C | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus mycoides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Bacillus mycoides | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | - |
Bacillus mycoides | ? | - |
? | |
| beta-glucan + H2O | - |
Bacillus mycoides S122C | ? | - |
? | |
| carboxymethylcellulose + H2O | best substrate | Bacillus mycoides | ? | - |
? | |
| carboxymethylcellulose + H2O | best substrate | Bacillus mycoides S122C | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Bacillus mycoides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CMcellulase | - |
Bacillus mycoides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus mycoides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | - |
Bacillus mycoides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus mycoides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | more than 60% of maximum activity | Bacillus mycoides |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
