Any feedback?
Literature summary for 3.2.1.4 extracted from
- Characterization of a halostable endoglucanase with organic solvent-tolerant property from Haloarcula sp. G10 (2013), Int. J. Biol. Macromol., 62, 101-106.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Benzene | enhances enzyme activity | Haloarcula sp. |  |
| n-hexane | enhances enzyme activity | Haloarcula sp. | |
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | considering its thermostable, alkali-stable, halostable and organic solvent-tolerant properties, the enzyme might be potentially useful for future applications in biotechnological processes | Haloarcula sp. |
General Stability
| General Stability | Organism |
|---|---|
| halostable, the enzyme retains more than 80% activity after incubation with 1030% NaCl for 24 h | Haloarcula sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl dicarbonate | 10 mM, 17.6% residual activity | Haloarcula sp. | |
| EDTA | 10 mM, 11.2% residual activity | Haloarcula sp. | |
| Hg2+ | 5 mM, 58% inhibition | Haloarcula sp. | |
| phenylmethylsulfonyl fluoride | 10 mM, 18.1% residual activity | Haloarcula sp. | |
| SDS | 10 mM, 49.2% residual activity | Haloarcula sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
cellulose | Km-value for carboxymethylcellulose-Na as substrate is 3.01 mg/ml | Haloarcula sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Haloarcula sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 1.3fold stimulation | Haloarcula sp. | |
| Mg2+ | 5 mM, 1.5fold stimulation | Haloarcula sp. | |
| NaCl | optimal activity at 17.5% NaCl | Haloarcula sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration | Haloarcula sp. |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 1-butanol | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| 1-decanol | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| Acetone | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| acetonitrile | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| benzene | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| chloroform | no effect on stability | Haloarcula sp. |
| cyclohexane | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| DMSO | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| Ethanol | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| isooctane | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| Methanol | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| additional information | high activity and stability in the presence of non-polar hydrophobic organic solvents | Haloarcula sp. |
| n-hexane | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| phenylmethylsulfonyl fluoride | decreases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
| toluene | increases stability of the enzyme as compared to activity in the absence of organic solvents | Haloarcula sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloarcula sp. | - |
- |
- |
| Haloarcula sp. G10 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloarcula sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 71.2 | - |
pH 9.0, 50°C | Haloarcula sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | - |
Haloarcula sp. | ? | - |
? | |
| cellulose + H2O | - |
Haloarcula sp. G10 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE | Haloarcula sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Haloarcula sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | 20°C: about 40% of maximal activity, 80°C: about 80% of maximal activity | Haloarcula sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
24 h incubation, enzyme retains more than 60% activity | Haloarcula sp. |
| 90 | - |
24 h incubation, complete loss of activity | Haloarcula sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Haloarcula sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | pH 6.0: about 80% of maximal activity, pH 11: about 70% of maximal activity | Haloarcula sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | good stability in the pH range from7.011.0 | Haloarcula sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
