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Literature summary for 3.2.1.4 extracted from

  • Bragatto, J.; Segato, F.; Cota, J.; Mello, D.B.; Oliveira, M.M.; Buckeridge, M.S.; Squina, F.M.; Driemeier, C.
    Insights on how the activity of an endoglucanase is affected by physical properties of insoluble celluloses (2012), J. Phys. Chem. B, 116, 6128-6136.
    View publication on PubMed
Search for more literature for 3.2.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 cellulose 13 celluloses characterized for crystallinity and crystal width (by X-ray diffraction), wet porosity (by thermoporometry), and particle size (by light scattering) Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35988
-
 x * 35988, calculated from sequence Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q9V2T0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose + H2O activity is evaluated on 13 insoluble celluloses characterized for crystallinity and crystal width (by X-ray diffraction), wet porosity (by thermoporometry), and particle size (by light scattering). Looser crystalline packing increases the lengths of released cello-oligomers as well as the maximum endoglucanase specific activity (kcat) Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35988, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
EglA
-
 Pyrococcus furiosus
endo-1,4-beta-glucanase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 assay at Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pyrococcus furiosus