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Literature summary for 3.2.1.4 extracted from

  • Kim, H.W.; Ishikawa, K.
    The role of disulfide bond in hyperthermophilic endocellulase (2013), Extremophiles, 17, 593-599.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method. The crystals are obtained over a period of about 3 days at 22°C Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
C106S melting temperature of the mutant enzyme is 2°C lower than the wild-type enzyme Pyrococcus horikoshii
P74C melting temperature of the mutant enzyme is 2°C lower than the wild-type enzyme Pyrococcus horikoshii
P74C/C106S melting temperature of the mutant enzyme is 2°C lower than the wild-type enzyme Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58925
-
-
Pyrococcus horikoshii OT-3 O58925
-
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
101
-
melting-temperature of the mutant enzymes P74C/C106S, P74C and C106S Pyrococcus horikoshii
103
-
wild type enzyme is thermally unfolded at 103.4°C Pyrococcus horikoshii