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Literature summary for 3.2.1.4 extracted from

  • El-Sersy, N.; Abd-Elnaby, H.; Abou-Elela, G.; Ibrahim, H.; El-Toukhy, N.
    Optimization, economization and characterization of cellulase produced by marine Streptomyces ruber (2010), Afr. J. Biotechnol., 9, 6355-6364.
No PubMed abstract available
Search for more literature for 3.2.1.4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE Streptomyces ruber
27000
-
 x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE Streptomyces ruber

Organism

Organism UniProt Comment Textmining
Streptomyces ruber
-
 contains two different types of CMCases type I and II
-

Purification (Commentary)

Purification (Comment) Organism
isolation and separation of two different types of CMCases, type I and II, by anion exchange chromatography Streptomyces ruber

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimization of culture conditions for production of carboxymethyl cellulase using Plackett-Burman design. Optimized conditions include high concentration of KH2PO4, low concentration of MgSO4, and rice straw at 30 g/l as low cost source of cellulose Streptomyces ruber
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
846.8
-
 purified type II CMCase, pH 4.8, 40°C Streptomyces ruber
4240
-
 purified type I CMCase, pH 4.8, 40°C Streptomyces ruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Streptomyces ruber ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE Streptomyces ruber

Synonyms

Synonyms Comment Organism
Carboxymethyl cellulase
-
 Streptomyces ruber
CMCase
-
 Streptomyces ruber

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Streptomyces ruber

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 activity range Streptomyces ruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces ruber

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range Streptomyces ruber