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Literature summary for 3.2.1.4 extracted from
- An acidic and thermostable carboxymethyl cellulase from the yeast Cryptococcus sp. S-2: purification, characterization and improvement of its recombinant enzyme production by high cell-density fermentation of Pichia pastoris (2008), Protein Expr. Purif., 60, 140-146.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | due to its acidophilic (pH 3.5) and high temperature stability (up to 90°C), CSCMCase may be useful for industrial application such as animal feed industry and clarification of fruit juices | Cryptococcus sp. S-2 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Cryptococcus sp. S-2 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
SDS-PAGE | Cryptococcus sp. S-2 |
| 34070 | - |
calculated | Cryptococcus sp. S-2 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cryptococcus sp. S-2 | A4UU22 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity from the supernatant by ultrafiltration, DEAE-5PW anion exchange column and TSK-gel G3000SW gel filtration | Cryptococcus sp. S-2 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.05 | - |
CM cellulose | Cryptococcus sp. S-2 |
| 4.93 | - |
carboxymethyl cellulose sodium salt | Cryptococcus sp. S-2 |
| 248.7 | - |
recombinant CSCMCase | Cryptococcus sp. S-2 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Cryptococcus sp. S-2 | ? | - |
? |  |
| cellobiose + H2O | - |
Cryptococcus sp. S-2 | ? | - |
? | |
| CM cellulose + H2O | - |
Cryptococcus sp. S-2 | ? | - |
? | |
| additional information | carboxymethyl cellulose sodium salt is a better substrate than insoluble cellulose substrate such as Avicel (0 U/mg), SIGMACELL cellulose (0 U/mg), and CM cellulose | Cryptococcus sp. S-2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Cryptococcus sp. S-2 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Carboxymethyl cellulase | - |
Cryptococcus sp. S-2 |
| CSCMCase | - |
Cryptococcus sp. S-2 |
| thermostable carboxymethyl cellulase | - |
Cryptococcus sp. S-2 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | - |
Cryptococcus sp. S-2 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
enzyme retains approximately 50% of its maximum activity after incubating at 90°C for 1 h | Cryptococcus sp. S-2 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
maximum carboxymethyl cellulase activity at | Cryptococcus sp. S-2 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
Cryptococcus sp. S-2 |
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Release 2023.1 (January 2023)
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