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Literature summary for 3.2.1.4 extracted from
- Hydrolysis of amorphous and crystalline cellulose by heterologously produced cellulases of Melanocarpus albomyces (2008), J. Biotechnol., 136, 140-147.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Trichoderma reesei | Melanocarpus albomyces |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | very low activity against 4-methyl-umberriferyl-beta-D-lactoside is almost totally inhibited by 0.005 M cellobiose | Melanocarpus albomyces |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
- |
Melanocarpus albomyces |
| 44830 | - |
calculated | Melanocarpus albomyces |
| 47550 | - |
calculated | Melanocarpus albomyces |
| 50000 | - |
- |
Melanocarpus albomyces |
| 50000 | - |
SDS-PAGE | Melanocarpus albomyces |
| 50020 | - |
calculated, with cellulose-binding module | Melanocarpus albomyces |
| 53780 | - |
calculated, with cellulose-binding module | Melanocarpus albomyces |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Melanocarpus albomyces | Q8J0K6 | - |
- |
| Melanocarpus albomyces | Q8J0K7 | - |
- |
| Melanocarpus albomyces | Q8J0K8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Melanocarpus albomyces |
| 50°C, pH 6.0 | Melanocarpus albomyces |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-beta-D-lactoside + H2O | very low activity | Melanocarpus albomyces | 4-methylumbelliferone + D-lactose | - |
? | |
| avicel + H2O | - |
Melanocarpus albomyces | ? | - |
? | |
| carboxymethylcellulose + H2O | - |
Melanocarpus albomyces | ? | - |
? | |
| cellulose + H2O | - |
Melanocarpus albomyces | ? | - |
? | |
| cellulose + H2O | crystalline and amorphous cellulose | Melanocarpus albomyces | ? | - |
? | |
| hydroxyethylcellulose + H2O | - |
Melanocarpus albomyces | ? | - |
? | |
| additional information | Cel7B has greater activity than the endoglucanases Cel45A and Cel7A against crystalline cellulose, whereas in the case of amorphous substrate the order is reversed | Melanocarpus albomyces | ? | - |
? | |
| phosphoric acid swollen cellulose + H2O | - |
Melanocarpus albomyces | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel45A | - |
Melanocarpus albomyces |
| Cel7A | - |
Melanocarpus albomyces |
| Cel7B | - |
Melanocarpus albomyces |
| Cellulase | - |
Melanocarpus albomyces |
| endoglucanase | - |
Melanocarpus albomyces |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Melanocarpus albomyces |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Melanocarpus albomyces |
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