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Literature summary for 3.2.1.4 extracted from
- Practical application of different enzymes immobilized on sepabeads (2008), Bioprocess Biosyst. Eng., 31, 163-171.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | Production of immobilized enzyme on Sepabeads EC-BU (hydrophobic interactions are the driving force for the adsorption of the enzyme to the carrier) | Pseudomonas putida |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| The gene encoding for the thermoactive endoglucanase is derived from a metagenomic gene library based on a mixed culture grown anaerobically on cellulose at 70 C and pH 7. The recombinant endoglucanase is actively expressed in Escherichia coli TunerTM(DE3)pLacI by induction with 1 mM IPTG. | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
- |
Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas putida | beta-1,4-endoglucanases show activities of the cleavage of the cellulose backbone due to an endocleavage. Oligosaccharides of different lengths as well as cellobiose and glucose are formed | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni-NTA column purification and pretreatment by heat denaturation to remove accompanying proteins | Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | metagenome | Pseudomonas putida | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | beta-1,4-endoglucanases show activities of the cleavage of the cellulose backbone due to an endocleavage. Oligosaccharides of different lengths as well as cellobiose and glucose are formed | Pseudomonas putida | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoglucanase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
enzyme is thermoactive | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
free enzyme shows an activity of 75% of the initial activity after 30 min, and even an activity of 40% remained after 6 h, both measured at 70°C | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudomonas putida |
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