Literature summary for 3.2.1.4 extracted from

Rudsander, U.J.; Sandstrom, C.; Piens, K.; Master, E.R.; Wilson, D.B.; Brumer Iii, H.; Kenne, L.; Teeri, T.T.
Comparative NMR analysis of cellooligosaccharide hydrolysis by GH9 bacterial and plant endo-1,4-beta-glucanases (2008), Biochemistry, 47, 5235-5241.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Thermobifida fusca
-	Populus tremula x Populus tremuloides

Protein Variants

Protein Variants	Comment	Organism
W209S	active site mutant	Thermobifida fusca
W256A	active site mutant	Thermobifida fusca
W313G	active site mutant	Thermobifida fusca

Organism

Organism	UniProt	Comment	Textmining
Populus tremula x Populus tremuloides	-	-	-
Thermobifida fusca	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermobifida fusca
-	Populus tremula x Populus tremuloides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.018	-	TfCel9A wild-type, activity on 600 microM G5	Thermobifida fusca
0.037	-	TfCel9A W256A mutant, activity on 600 microM G5	Thermobifida fusca

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellooligosaccharide + H2O	-	Thermobifida fusca	?	-	?
cellooligosaccharide + H2O	-	Populus tremula x Populus tremuloides	?	-	?

Synonyms

Synonyms	Comment	Organism
Cel9A	-	Populus tremula x Populus tremuloides
cellulase Cel9A	-	Thermobifida fusca
endo-1,4-beta-glucanase	-	Thermobifida fusca
endo-1,4-beta-glucanase	-	Populus tremula x Populus tremuloides
endoglucanase	-	Thermobifida fusca
endoglucanase	-	Populus tremula x Populus tremuloides
PttCel9A	-	Populus tremula x Populus tremuloides
TfCel9A	-	Thermobifida fusca

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Release 2023.1 (January 2023)