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Literature summary for 3.2.1.4 extracted from

  • Jabbar, A.; Rashid, M.H.; Javed, M.R.; Perveen, R.; Malana, M.A.
    Kinetics and thermodynamics of a novel endoglucanase (CMCase) from Gymnoascella citrina produced under solid-state condition (2008), J. Ind. Microbiol. Biotechnol., 35, 515-524.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Gymnascella citrina
Mn2+ above 5 mM Gymnascella citrina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KM-value for carboxymethylcellulase is 6.25 mg/ml at pH 5.0, 50°C Gymnascella citrina

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the removal of metals results into complete loss of enzymatic activity and is completely recovered in the presence of 1 mM Mn2+. 12fold activation at 1.5 mM Mn2+ Gymnascella citrina
additional information no inhibition up to 7 mM of Ca2+, Zn2+ and K+ is observed Gymnascella citrina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
1 * 42000, SDS-PAGE Gymnascella citrina
43000
-
gel filtratiion Gymnascella citrina

Organism

Organism UniProt Comment Textmining
Gymnascella citrina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gymnascella citrina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
115
-
-
Gymnascella citrina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Gymnascella citrina ?
-
?
carboxymethyl cellulose + H2O
-
Gymnascella citrina D-glucose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Gymnascella citrina

Synonyms

Synonyms Comment Organism
CMCase-I
-
Gymnascella citrina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Gymnascella citrina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
318
-
carboxymethyl cellulose in presence of 1.5 mM Mn2+ Gymnascella citrina

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 6.5 stable Gymnascella citrina