Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
C159A | kcat/KM for p-nitrophenyl cellobiose is 1.8fold lower than wild-type value | Pyrococcus horikoshii |
E163A | kcat/KM for p-nitrophenyl cellobiose is 6fold lower than wild-type value | Pyrococcus horikoshii |
G158A | kcat/KM for p-nitrophenyl cellobiose is 2fold lower than wild-type value | Pyrococcus horikoshii |
H155A | kcat/KM for p-nitrophenyl cellobiose is 140fold lower than wild-type value | Pyrococcus horikoshii |
H161A | kcat/KM for p-nitrophenyl cellobiose is neatrly identical to wild-type value | Pyrococcus horikoshii |
I157A | kcat/KM for p-nitrophenyl cellobiose is nearly identical to wild-type value | Pyrococcus horikoshii |
I162A | kcat/KM for p-nitrophenyl cellobiose is 140fold lower than wild-type value | Pyrococcus horikoshii |
P164A | kcat/KM for p-nitrophenyl cellobiose is 6fold lower than wild-type value | Pyrococcus horikoshii |
R156A | kcat/KM for p-nitrophenyl cellobiose is 10fold lower than wild-type value | Pyrococcus horikoshii |
T160A | kcat/KM for p-nitrophenyl cellobiose is nearly identical to wild-type value | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme I157A | Pyrococcus horikoshii | |
0.92 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme T160A | Pyrococcus horikoshii | |
1.12 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, wild-type enzyme | Pyrococcus horikoshii | |
1.23 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme H161A | Pyrococcus horikoshii | |
1.37 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme G158A | Pyrococcus horikoshii | |
1.55 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme E163A | Pyrococcus horikoshii | |
1.58 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme P164A | Pyrococcus horikoshii | |
1.9 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme C159A | Pyrococcus horikoshii | |
4.57 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme I162A | Pyrococcus horikoshii | |
5.85 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme R156A | Pyrococcus horikoshii | |
6.69 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme H155A | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58925 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | - |
Pyrococcus horikoshii | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Pyrococcus horikoshii | ? | - |
? | |
crystalline cellulase + H2O | - |
Pyrococcus horikoshii | ? | - |
? | |
p-nitrophenyl cellobiose + H2O | - |
Pyrococcus horikoshii | p-nitrophenol + cellobiose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme I162A | Pyrococcus horikoshii | |
0.006 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme H155A | Pyrococcus horikoshii | |
0.036 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme E163A | Pyrococcus horikoshii | |
0.037 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme P164A | Pyrococcus horikoshii | |
0.083 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme R156A | Pyrococcus horikoshii | |
0.092 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme G158A | Pyrococcus horikoshii | |
0.115 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme I157A | Pyrococcus horikoshii | |
0.129 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme T160A | Pyrococcus horikoshii | |
0.144 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme C159A | Pyrococcus horikoshii | |
0.157 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, wild-type enzyme | Pyrococcus horikoshii | |
0.167 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme H161A | Pyrococcus horikoshii |