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Literature summary for 3.2.1.4 extracted from

  • Bukhtojarov, F.E.; Ustinov, B.B.; Salanovich, T.N.; Antonov, A.I.; Gusakov, A.V.; Okunev, O.N.; Sinitsyn, A.P.
    Cellulase complex of the fungus Chrysosporium lucknowense: isolation and characterization of endoglucanases and cellobiohydrolases (2004), Biochemistry, 69, 542-551.
    View publication on PubMed
Search for more literature for 3.2.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM-value for carboxymethyl cellulase is 1.3 g/l, KM-value for beta-glucan is 1.14 g/l, endoglucanase EG44 Chrysosporium lucknowense
additional information
-
 additional information KM-value for carboxymethyl cellulase is 11 g/l, KM-value for beta-glucan is 0.12 g/l, endoglucanase EG28 Chrysosporium lucknowense
additional information
-
 additional information KM-value for carboxymethyl cellulase is 5.4 g/l, KM-value for beta-glucan is 3.0 g/l, endoglucanase EG25 Chrysosporium lucknowense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, endoglucanase EG25, SDS-PAGE Chrysosporium lucknowense
28000
-
 x * 28000, endoglucanase EG28, SDS-PAGE Chrysosporium lucknowense
44000
-
 x * 44000, endoglucanase EG44, SDS-PAGE Chrysosporium lucknowense

Organism

Organism UniProt Comment Textmining
Chrysosporium lucknowense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
endoglucanase EG25 Chrysosporium lucknowense
endoglucanase EG28 Chrysosporium lucknowense
endoglucanase EG44 Chrysosporium lucknowense

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Chrysosporium lucknowense
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O endoglucanase EG25 Chrysosporium lucknowense ?
-
 ?
beta-glucan + H2O endoglucanase EG28 Chrysosporium lucknowense ?
-
 ?
beta-glucan + H2O endoglucanase EG44 Chrysosporium lucknowense ?
-
 ?
carboxymethyl cellulose + H2O endoglucanase EG25 Chrysosporium lucknowense ?
-
 ?
carboxymethyl cellulose + H2O endoglucanase EG28 Chrysosporium lucknowense ?
-
 ?
carboxymethyl cellulose + H2O endoglucanase EG44 Chrysosporium lucknowense ?
-
 ?
p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG25 Chrysosporium lucknowense ?
-
 ?
p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG28 Chrysosporium lucknowense ?
-
 ?
p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG44 Chrysosporium lucknowense ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, endoglucanase EG25, SDS-PAGE Chrysosporium lucknowense
? x * 28000, endoglucanase EG28, SDS-PAGE Chrysosporium lucknowense
? x * 44000, endoglucanase EG44, SDS-PAGE Chrysosporium lucknowense

Synonyms

Synonyms Comment Organism
EG25
-
 Chrysosporium lucknowense
EG28
-
 Chrysosporium lucknowense
EG44 EG44 and RG51 are different forms of one enzyme. EG44 seems to be the catalytic module of an intact EG51 without a cellulose-binding module Chrysosporium lucknowense
endoglucanase EG25
-
 Chrysosporium lucknowense
endoglucanase EG28
-
 Chrysosporium lucknowense
endoglucanase EG44 endoglucanase EG44 and endoglucanase EG51 are different forms of one enzyme. EG44 seems to be the catalytic module of an intact EG51 without a cellulose-binding module Chrysosporium lucknowense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 endoglucanase EG28 Chrysosporium lucknowense
65
-
 endoglucanase EG25 Chrysosporium lucknowense
70
-
 endoglucanase EG44 Chrysosporium lucknowense

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 30°C: about 55% of maximal activity, 75°C: about 60% of maximal activity, endoglucanase EG28 Chrysosporium lucknowense
45 75 45°C: about 45% of maximal activity, 75°C: about 60% of maximal activity, endoglucanase EG25 Chrysosporium lucknowense
50 75 50°C: about 35% of maximal activity, 75°C: about 60% of maximal activity, endoglucanase EG44 Chrysosporium lucknowense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 beta-Glucan 50°C, pH 5.5, endoglucanase EG25 Chrysosporium lucknowense
15
-
 carboxymethyl cellulose 50°C, pH 5.5, endoglucanase EG25 Chrysosporium lucknowense
19
-
 carboxymethyl cellulose 50°C, pH 5.5, endoglucanase EG28 Chrysosporium lucknowense
54
-
 carboxymethyl cellulose 50°C, pH 5.5, endoglucanase EG44 Chrysosporium lucknowense
64
-
 beta-Glucan 50°C, pH 5.5, endoglucanase EG28 Chrysosporium lucknowense
76
-
 beta-Glucan 50°C, pH 5.5, endoglucanase EG44 Chrysosporium lucknowense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Chrysosporium lucknowense

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8.5 pH 3.5: about 50% of maximal activity, pH 8.5: about 55% of maximal activity, endoglucanase EG25 Chrysosporium lucknowense
4 7.5 pH 4.0: about 60% of maximal activity, pH 7.5: about 35% of maximal activity, endoglucanase EG28 Chrysosporium lucknowense
4 7.5 pH 4.0: about 75% of maximal activity, pH 7.5: about 65% of maximal activity, endoglucanase EG44 Chrysosporium lucknowense

pI Value

Organism Comment pI Value Maximum pI Value
Chrysosporium lucknowense isoelectric focusing, endoglucanase EG25
-
 4
Chrysosporium lucknowense isoelectric focusing, endoglucanase EG28
-
 5.7
Chrysosporium lucknowense isoelectric focusing, endoglucanase EG44
-
 6