Literature summary for 3.2.1.4 extracted from

Larsson, A.M.; Bergfors, T.; Dultz, E.; Irwin, D.C.; Roos, A.; Driguez, H.; Wilson, D.B.; Jones, T.A.
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion (2005), Biochemistry, 44, 12915-12922.

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor-diffusion method at room temperature. Structure of the complex of the catalytic domain of endoglucanase Cel6A with a nonhydrolyzable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside determined at 1.5 A resolution, structure of mutant enzyme Y73S, structure of mutant enzyme Y73S in complex with methylcellobiosyl-4-thio-beta-cellobioside at 1.04 A resolution, structure of mutant enzyme Y73S in complex with cellotetrose at 1.64 A resolution	Thermobifida fusca

Crystallization (Comment)

Organism

hanging-drop vapor-diffusion method at room temperature. Structure of the complex of the catalytic domain of endoglucanase Cel6A with a nonhydrolyzable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside determined at 1.5 A resolution, structure of mutant enzyme Y73S, structure of mutant enzyme Y73S in complex with methylcellobiosyl-4-thio-beta-cellobioside at 1.04 A resolution, structure of mutant enzyme Y73S in complex with cellotetrose at 1.64 A resolution

Thermobifida fusca

Protein Variants

Protein Variants	Comment	Organism
D117A	activity with carboxymethyl cellulose is 0.03% of wild-type activity, activity with phosphoric acid-swollen cellulose is 0.02% of wild-type activity	Thermobifida fusca
Y73F	activity with carboxymethyl cellulose is 8.4% of wild-type activity, activity with phosphoric acid-swollen cellulose is 5.7% of wild-type activity	Thermobifida fusca
Y73S	activity with carboxymethyl cellulose is 0.022% of wild-type activity, activity with phosphoric acid-swollen cellulose is 0.088% of wild-type activity	Thermobifida fusca

Organism

Organism	UniProt	Comment	Textmining
Thermobifida fusca	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carboxymethyl cellulose + H2O	-	Thermobifida fusca	?	-	?
phosphoric acid-swollen cellulose + H2O	-	Thermobifida fusca	?	-	?

Synonyms

Synonyms	Comment	Organism
endoglucanase Cel6A	-	Thermobifida fusca