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Literature summary for 3.2.1.4 extracted from

  • Matsumoto, K.; Endo, Y.; Tamiya, N.; Kano, M.; Miyauchi, K.; Abe, J.
    Studies on cellulase produced by the phytopathogens. Purification and enzymatic properties of cellulase of Fusarium moniliforme (1974), J. Biochem., 76, 563-572.
    View publication on PubMed
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid 2 mM, slight activation, cellulase I Fusarium verticillioides
hydroquinone 2 mM, slight activation, cellulase I Fusarium verticillioides

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 2 mM, cellulase I Fusarium verticillioides
mercaptoethanol 1 mM, slight inhibition Fusarium verticillioides
Mg2+ 2 mM, slight inhibition Fusarium verticillioides
N-bromosuccinimide
-
 Fusarium verticillioides
Pb2+ 2 mM, slight inhibition Fusarium verticillioides
sodium picryl sulfate 2 mM, cellulase I Fusarium verticillioides

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 2 mM, slight activation, cellulase I Fusarium verticillioides
ZnCl2 2 mM, slight activation, cellulase I Fusarium verticillioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 cellulase I, gel filtration Fusarium verticillioides

Organism

Organism UniProt Comment Textmining
Fusarium verticillioides
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein cellulase I contains about 26% carbohydrate Fusarium verticillioides

Purification (Commentary)

Purification (Comment) Organism
cellulase I and II Fusarium verticillioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O cellulase I Fusarium verticillioides ?
-
 ?
carboxymethylcellulose + H2O
-
 Fusarium verticillioides additional information
-
 ?
additional information hydrolysis of filter paper Fusarium verticillioides ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 cellulase I Fusarium verticillioides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 65% of maximal activity, 80°C: about 90% of maximal activity Fusarium verticillioides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 24 h, stable Fusarium verticillioides
37
-
 3 h, stable Fusarium verticillioides
60
-
 120 min, little loss of activity Fusarium verticillioides
80
-
 30 min, 50% loss of activity Fusarium verticillioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 cellulase I Fusarium verticillioides

pH Range

pH Minimum pH Maximum Comment Organism
3.6 8 pH 3.6: about 65% of maximal activity, about 55% of maximal activity Fusarium verticillioides

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 cellulase I is stable Fusarium verticillioides