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Literature summary for 3.2.1.4 extracted from

  • Okada, G.
    Enzymatic studies on a cellulase system of Trichoderma viride. IV. Purification and properties of a less-random type cellulase (1976), J. Biochem., 80, 913-922.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, partial inhibition Trichoderma viride
Cu2+ 1 mM, partial inhibition Trichoderma viride
Hg2+ 1 mM, complete inactivation Trichoderma viride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Trichoderma viride
0.45
-
cellohexaose
-
Trichoderma viride
0.56
-
cellopentaose
-
Trichoderma viride
0.67
-
cellotetraose
-
Trichoderma viride
2.94
-
cellotriose
-
Trichoderma viride

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Trichoderma viride

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cellulase III Trichoderma viride

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 cellobiose transfer reaction Trichoderma viride cellotetraose + H2O
-
?
avicel + H2O
-
Trichoderma viride ?
-
?
carboxymethylcellulose + H2O
-
Trichoderma viride additional information
-
?
cellohexaose + H2O
-
Trichoderma viride additional information
-
?
cellopentaose + H2O
-
Trichoderma viride additional information
-
?
cellotetraose + H2O
-
Trichoderma viride 2 cellobiose
-
?
cellotriose + H2O
-
Trichoderma viride D-glucose + cellobiose
-
?
additional information hydrolysis of cotton Trichoderma viride ?
-
?
additional information hydrolysis of filter paper Trichoderma viride ?
-
?
p-nitrophenyl beta-D-cellobioside + H2O cellulase III preferentially attacks the aglycone linkage Trichoderma viride glucose + cellobiose + ?
-
?

Synonyms

Synonyms Comment Organism
Meicelase
-
Trichoderma viride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Trichoderma viride

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
pH 4.5-5.5, stable Trichoderma viride
100
-
10 min, 60% loss of activity Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 cellulase III Trichoderma viride

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 4°C, 24 h, stable Trichoderma viride