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Literature summary for 3.2.1.4 extracted from

  • Fukumori, F.; Kudo, T.; Horikoshi, K.
    Purification and properties of a cellulase from alkalophilic Bacillus sp. No. 1139 (1985), J. Gen. Microbiol., 131, 3339-3345.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM CdCl2, complete inhibition Bacillus sp. (in: Bacteria)
Co2+ 1 mM CoCl2, 44% inhibition Bacillus sp. (in: Bacteria)
Cu2+ 1 mM CuCl2, 41% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 1 mM HgCl2, complete inhibition Bacillus sp. (in: Bacteria)
Ni2+ 1 mM NiCl2, 68% inhibition Bacillus sp. (in: Bacteria)
Pb2+ 1 mM Pb(CH3COO)-, 36% inhibition Bacillus sp. (in: Bacteria)
Zn2+ 1 mM ZnSO4, 88% inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 25 mM, activation Bacillus sp. (in: Bacteria)
NaCl 25 mM, activation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
No. 1139
-
Bacillus sp. (in: Bacteria) No. 1139
-
No. 1139
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.9
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Bacillus sp. (in: Bacteria) additional information
-
?
carboxymethylcellulose + H2O
-
Bacillus sp. (in: Bacteria) No. 1139 additional information
-
?
cellotetraose + H2O
-
Bacillus sp. (in: Bacteria) 2 cellobiose glucose, 15%, cellobiose, 64%, cellotriose, 14% and cellotetraose, 3.3% ?
cellotetraose + H2O
-
Bacillus sp. (in: Bacteria) No. 1139 2 cellobiose glucose, 15%, cellobiose, 64%, cellotriose, 14% and cellotetraose, 3.3% ?
cellotriose + H2O
-
Bacillus sp. (in: Bacteria) D-glucose + cellobiose a mixture of glucose, 5.5%, cellobiose, 55%, cellotriose, 34%, and traces of cellotetraose ?
cellotriose + H2O
-
Bacillus sp. (in: Bacteria) No. 1139 D-glucose + cellobiose a mixture of glucose, 5.5%, cellobiose, 55%, cellotriose, 34%, and traces of cellotetraose ?
additional information trans-glucosidase activity also observed Bacillus sp. (in: Bacteria) ?
-
?
additional information trans-glucosidase activity also observed Bacillus sp. (in: Bacteria) No. 1139 ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min, stable up to Bacillus sp. (in: Bacteria)
50
-
10 min, 90% of the original activity is retained Bacillus sp. (in: Bacteria)
60
-
10 min, complete inactivation Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
7 11.5 pH 7.0: about 20% of maximal activity, pH 11.5: about 35% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 4°C, 24 h, stable Bacillus sp. (in: Bacteria)