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Literature summary for 3.2.1.4 extracted from
- Purification and properties of the endo-1,4-beta-glucanase from Bacillus subtilis (1987), J. Gen. Microbiol., 133, 2155-2162.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| arabitol | 50 mM, stimulates | Bacillus subtilis |  |
| dithiothreitol | 5 mM, stimulates | Bacillus subtilis | |
| erythritol | 50 mM, stimulates | Bacillus subtilis | |
| Gly | 50 mM, stimulates | Bacillus subtilis | |
| glycerol | 50 mM, stimulates | Bacillus subtilis | |
| histamine | 20 mM, stimulates | Bacillus subtilis | |
| L-His | 50 mM, stimulates | Bacillus subtilis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Bacillus subtilis | |
| Cu2+ | - |
Bacillus subtilis | |
| EDTA | - |
Bacillus subtilis | |
| Fe2+ | - |
Bacillus subtilis | |
| Hg2+ | - |
Bacillus subtilis | |
| Pb2+ | - |
Bacillus subtilis | |
| Sn2+ | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | increases activity by 139.5% | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Bacillus subtilis | additional information | glucose + cellobiose | ? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
60 min, stable | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | 50°C, over 95% of the activity is retained | Bacillus subtilis |
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