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Literature summary for 3.2.1.4 extracted from

  • Beguin, P.; Joliff, G.; Juy, M.; Amit, A.G.; Millet, J.; Poljak, R.J.; Aubert, J.P.
    Crystalline endoglucanase D from Clostridium thermocellum overproduced in Escherichia coli (1988), Methods Enzymol., 160, 355-362.
No PubMed abstract available
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
overproduction in Escherichia coli Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 equilibrium sedimentation Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Acetivibrio thermocellus
315
-
 recombinant enzyme from cytoplasmic supernatant of Escherichia coli Acetivibrio thermocellus
428
-
 recombinant enzyme from granules produced by Escherichia coli Acetivibrio thermocellus

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 denaturation above Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Acetivibrio thermocellus

pH Range

pH Minimum pH Maximum Comment Organism
4.8 8.2 more than 50% of maximal activity at pH 4.8 and at pH 8.2 Acetivibrio thermocellus