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Literature summary for 3.2.1.4 extracted from

  • Wilson, D.B.
    Cellulases of Thermomonospora fusca (1988), Methods Enzymol., 160, 314-323.
No PubMed abstract available
Search for more literature for 3.2.1.4

Inhibitors

Inhibitors Comment Organism Structure
Ag+ AgNO3, endoglucanase E1 and E2 Thermobifida fusca
Hg2+ endoglucanase E1 and E2; HgCl2 Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme E2 and E3 are glycoproteins Thermobifida fusca
no modification enzyme E1, E4 and E5 are not glycoproteins Thermobifida fusca

Purification (Commentary)

Purification (Comment) Organism
enzyme form E1, E2, E3, E4 and E5 Thermobifida fusca

Storage Stability

Storage Stability Organism
-70°C, purified enzymes are very stable Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
 Thermobifida fusca additional information
-
 ?
cellotriose + H2O hydrolyzed by endoglucanase E1, no activity with endoglucanase E2 Thermobifida fusca D-glucose + cellobiose + glucose, endoglucanase E1 ?
cellulose + H2O amorphous cellulose Thermobifida fusca ? endoglucanase E1 produces mainly cellobiose and a trace of glucose, endoglucanase E2 produces mainly cellotriose and higher oligomers with some cellobiose and a trace of glucose ?
xylan + H2O
-
 Thermobifida fusca additional information
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 endoglucanase E1 and E2 Thermobifida fusca