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Literature summary for 3.2.1.4 extracted from

  • Voragen, A.G.J.; Beldman, G.; Rombouts, F.M.
    Cellulases of a mutant strain of Trichoderma viride QM 9414 (1988), Methods Enzymol., 160, 243-251.
No PubMed abstract available
Search for more literature for 3.2.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Trichoderma viride

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, endoglucanase II, SDS-PAGE Trichoderma viride
50000
-
 x * 50000, endoglucanase IV, SDS-PAGE Trichoderma viride
50000
-
 x * 50000, endoglucanase I, SDS-PAGE Trichoderma viride
52000
-
 x * 52000, endoglucanase VI, SDS-PAGE Trichoderma viride
57000
-
 x * 57000, endoglucanase V, SDS-PAGE Trichoderma viride
585000
-
 x * 585000, endoglucanase III, SDS-PAGE Trichoderma viride

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
 QM 9414, mutant strain
-
Trichoderma viride QM 9414
-
 QM 9414, mutant strain
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein endoglucanases I, II, III, V, and VI are glycoproteins Trichoderma viride
no modification endoglucanase IV contains no carbohydrate Trichoderma viride

Purification (Commentary)

Purification (Comment) Organism
endoglucanase I, II, III, IV, V and VI Trichoderma viride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Trichoderma viride ?
-
 ?
carboxymethylcellulose + H2O
-
 Trichoderma viride additional information
-
 ?
cellulose + H2O acid-swollen Trichoderma viride ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, endoglucanase IV, SDS-PAGE Trichoderma viride
? x * 45000, endoglucanase II, SDS-PAGE Trichoderma viride
? x * 52000, endoglucanase VI, SDS-PAGE Trichoderma viride
? x * 57000, endoglucanase V, SDS-PAGE Trichoderma viride
? x * 585000, endoglucanase III, SDS-PAGE Trichoderma viride
? x * 50000, endoglucanase I, SDS-PAGE Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 endoglucanase II Trichoderma viride
4.5
-
 endoglucanase V and VI Trichoderma viride
5
-
 endoglucanase I and III Trichoderma viride
5.5
-
 endoglucanase IV Trichoderma viride