Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxy-5-nitrobenzylbromide | 47% inhibition at 5 mM | Laceyella putida | |
Hg2+ | 39% inhibition at 1 mM | Laceyella putida | |
monoiodoacetate | 34% inhibition at 5 mM | Laceyella putida | |
Pd2+ | 23% inhibition at 1 mM | Laceyella putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Laceyella putida | A0A0P0UPC6 | - |
- |
Laceyella putida JAM FM3001 | A0A0P0UPC6 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography, butyl-Toyopearl column chromatography, and phenyl-Toyopearl column chromatography | Laceyella putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
curdlan + H2O | 100% activity | Laceyella putida | laminaribiose + laminaritriose | - |
? | |
curdlan + H2O | 100% activity | Laceyella putida JAM FM3001 | laminaribiose + laminaritriose | - |
? | |
laminarin + H2O | 47.6% activity compared to curdlan | Laceyella putida | ? | - |
? | |
laminarin + H2O | 47.6% activity compared to curdlan | Laceyella putida JAM FM3001 | ? | - |
? | |
additional information | no activity with xylan, colloidal chitin, carboxymethylcellulose, polygalacturonic acid, and pectin | Laceyella putida | ? | - |
? | |
additional information | no activity with xylan, colloidal chitin, carboxymethylcellulose, polygalacturonic acid, and pectin | Laceyella putida JAM FM3001 | ? | - |
? | |
pachyman + H2O | most favored substrate, 223% activity compared to curdlan | Laceyella putida | ? | - |
? | |
pachyman + H2O | most favored substrate, 223% activity compared to curdlan | Laceyella putida JAM FM3001 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Laceyella putida |
? | x * 39836, calculated from amino acid sequence | Laceyella putida |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3 glucanase | - |
Laceyella putida |
GluA | - |
Laceyella putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Laceyella putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | the enzyme activity is drastically elevated above 40°C and reaches the maximum level at 80°C in 100 mM glycine-HCl buffer (pH 4.2) | Laceyella putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 75 | the enzyme is stable during 30 min incubation from 30 to 75°C, where the maximal residual activity is 35 to 45% of the original activity | Laceyella putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
- |
Laceyella putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | the enzyme shows more than 60% activity between pH 3.0 and 7.0 and does not work well at pH 8.0 or above | Laceyella putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.4 | 8.7 | when the enzyme is incubated at 40°C for 30 min, the enzyme is stable over the pH range of 2.4 to 8.7 | Laceyella putida |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Laceyella putida | isoelectric focusing | - |
5 |
Organism | Comment | Expression |
---|---|---|
Laceyella putida | enzyme expression is induced by N-acetylglucosamine | up |