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Literature summary for 3.2.1.39 extracted from

  • Cota, J.; Alvarez, T.M.; Citadini, A.P.; Santos, C.R.; de Oliveira Neto, M.; Oliveira, R.R.; Pastore, G.M.; Ruller, R.; Prade, R.A.; Murakami, M.T.; Squina, F.M.
    Mode of operation and low-resolution structure of a multi-domain and hyperthermophilic endo-beta-1,3-glucanase from Thermotoga petrophila (2011), Biochem. Biophys. Res. Commun., 406, 590-594.
    View publication on PubMed
Search for more literature for 3.2.1.39

Crystallization (Commentary)

Crystallization (Comment) Organism
small angle X-ray scattering data and homology modeling. Enzyme displays a multi-domain structural architecture of a V-shape envelope with a catalytic domain flanked by two carbohydrate-binding modules Thermotoga petrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km value for laminarin is 3.3 mg/ml Thermotoga petrophila

Organism

Organism UniProt Comment Textmining
Thermotoga petrophila A5IL44 member of glycosyl hydrolase family 16
-
Thermotoga petrophila RKU-1 A5IL44 member of glycosyl hydrolase family 16
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.4
-
 substrate lichenan, pH 6.0, 91°C Thermotoga petrophila
41.55
-
 substrate beta-glucan, pH 6.0, 91°C Thermotoga petrophila
48.1
-
 substrate laminarin, pH 6.0, 91°C Thermotoga petrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucan + H2O
-
 Thermotoga petrophila ?
-
 ?
beta-D-glucan + H2O
-
 Thermotoga petrophila RKU-1 ?
-
 ?
laminarin + H2O
-
 Thermotoga petrophila D-glucose + laminaribiose + laminaritriose major products, enzyme most frequently attacks the bond between the 3rd and 4th residue from the non-reducing end ?
laminarin + H2O
-
 Thermotoga petrophila RKU-1 D-glucose + laminaribiose + laminaritriose major products, enzyme most frequently attacks the bond between the 3rd and 4th residue from the non-reducing end ?
lichenan + H2O
-
 Thermotoga petrophila ?
-
 ?
lichenan + H2O
-
 Thermotoga petrophila RKU-1 ?
-
 ?
additional information specific cleavage of internal beta-1,3-bonds. No substrate: avicel, carboxymethyl cellulose and cellopentaose, xylan, arabinan and manan containing polysaccharides Thermotoga petrophila ?
-
 ?
additional information specific cleavage of internal beta-1,3-bonds. No substrate: avicel, carboxymethyl cellulose and cellopentaose, xylan, arabinan and manan containing polysaccharides Thermotoga petrophila RKU-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
LAM
-
 Thermotoga petrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
91
-
-
 Thermotoga petrophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
78
-
 pH 6.0, 82% of maximum activity Thermotoga petrophila
95
-
 pH 6.0, 86% of maximum activity Thermotoga petrophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable Thermotoga petrophila
80
-
 16 h, 60% residual activity Thermotoga petrophila
90
-
 half-life 577 min Thermotoga petrophila
95
-
 half-life 126 min Thermotoga petrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
680.3
-
 Laminarin pH 6.0, 91°C Thermotoga petrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Thermotoga petrophila

pH Range

pH Minimum pH Maximum Comment Organism
5 7 more than 80% of maximum activity Thermotoga petrophila