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Literature summary for 3.2.1.39 extracted from
- Characterization of cultivar differences in beta-1,3 glucanase gene expression, glucanase activity and fruit pulp softening rates during fruit ripening in three naturally occurring banana cultivars (2009), Plant Cell Rep., 28, 1641-1653.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning from cultivars Rasthali, Kanthali, and Monthan, DNA and amino acid sequence determination and analysis, expression of His-tagged enzymes in Escherichia coli strain M15 | Musa x paradisiaca |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Musa x paradisiaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Musa x paradisiaca | - |
different cultivars Rasthali, Kanthali, and Monthan | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzymes from Escherichia coli strain M15 by nickel affinity chromatography | Musa x paradisiaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | cultivar differences in beta-1,3 glucanase gene expression, glucanase activity and fruit pulp softening rates during fruit ripening in three naturally occurring banana cultivars. Low enzyme expressiong during fruit ripening | Musa x paradisiaca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0025 | 0.015 | activites of purified recombinant enzymes from different cultivars, overview | Musa x paradisiaca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | from cell wall polysaccharide extracts of fruits or from barley, activites and substrate specificities of enzymes from different cultivars, overview | Musa x paradisiaca | ? | - |
? |  |
| laminarin + H2O | - |
Musa x paradisiaca | ? | - |
? | |
| pachyman + H2O | - |
Musa x paradisiaca | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Musa x paradisiaca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3 glucanase | - |
Musa x paradisiaca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Musa x paradisiaca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 65 | purified recombinant enzyme, 30 min, stable | Musa x paradisiaca |
| 70 | - |
purified recombinant enzyme, 10 min, partly loss of activity | Musa x paradisiaca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
assay at | Musa x paradisiaca |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Musa x paradisiaca | ethylene treatment differentially stimulates beta-1,3 glucanase expression in the three banana cultivars, overview. Noinduction incv. Monthan, slight induction in cv. Kanthali, promninent induction in cv. Rasthali | additional information |
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