Cloned (Comment) | Organism |
---|---|
expression of the isolated wild-type and mutant LamA discoidin domains as soluble His-tagged proteins in Escherichia coli strain BL21(DE3) | Paenibacillus sp. CCRC 17245 |
Protein Variants | Comment | Organism |
---|---|---|
W1679A | site-directed mutagenesis, the mutation in the F5/8C module or DS domain decreases the protein affinity to laminarin | Paenibacillus sp. CCRC 17245 |
W1688A | site-directed mutagenesis, the mutation in the F5/8C module or DS domain decreases the protein affinity to laminarin | Paenibacillus sp. CCRC 17245 |
W1729A | site-directed mutagenesis, mutation in the F5/8C module or DS domain, the mutant domain forms inclusion bodies upon expression in Escherichia coli | Paenibacillus sp. CCRC 17245 |
Y1714A | site-directed mutagenesis, the mutation in the F5/8C module or DS domain decreases the protein affinity to laminarin | Paenibacillus sp. CCRC 17245 |
Y1768A | site-directed mutagenesis, the mutation in the F5/8C module or DS domain decreases the protein affinity to laminarin | Paenibacillus sp. CCRC 17245 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | Paenibacillus sp. CCRC 17245 | - |
? | - |
? | |
laminarin + H2O | Paenibacillus sp. CCRC 17245 BCRC | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. CCRC 17245 | Q000P7 | gene lamA | - |
Paenibacillus sp. CCRC 17245 BCRC | Q000P7 | gene lamA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant LamA discoidin domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Paenibacillus sp. CCRC 17245 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | i.e. microcrystalline cellulose | Paenibacillus sp. CCRC 17245 | ? | - |
? | |
avicel + H2O | i.e. microcrystalline cellulose | Paenibacillus sp. CCRC 17245 BCRC | ? | - |
? | |
chitin + H2O | - |
Paenibacillus sp. CCRC 17245 | ? | - |
? | |
chitin + H2O | - |
Paenibacillus sp. CCRC 17245 BCRC | ? | - |
? | |
laminarin + H2O | - |
Paenibacillus sp. CCRC 17245 | ? | - |
? | |
laminarin + H2O | - |
Paenibacillus sp. CCRC 17245 BCRC | ? | - |
? | |
lichenan + H2O | - |
Paenibacillus sp. CCRC 17245 | ? | - |
? | |
lichenan + H2O | - |
Paenibacillus sp. CCRC 17245 BCRC | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the beta-1,3-glucanase LamA is highly modular, containing a signal sequence, three repeats of the S-layer homologous module, a segment with unknown function, a catalytic module of glycoside hydrolase family 16, GH16, four repeats of CBM4 family, and a F5/8C module representing a discoidin domain, DS domain, from N- to C-terminus. The recombinant isolated purified His-tagged DS domain forms dimers | Paenibacillus sp. CCRC 17245 |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-glucanase | - |
Paenibacillus sp. CCRC 17245 |
LamA | - |
Paenibacillus sp. CCRC 17245 |
More | LamA contains a catalytic module of the glycoside hydrolase family 16, GH16 | Paenibacillus sp. CCRC 17245 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Paenibacillus sp. CCRC 17245 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
melting temperatures of wild-type and mutant DS domains between 52.3°C and 58.3°C | Paenibacillus sp. CCRC 17245 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
assay at | Paenibacillus sp. CCRC 17245 |