Cloned (Comment) | Organism |
---|---|
placing of gene eng2+ under the control of a repressible promoter | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a point mutation, that destroys the catalytic activity of the protein, results in a phenotype similar to that of the mutant strain with gene eng2 placed under the control of a repressible promoter. Exogenous addition of purified Eng2 releases the ascospores from asci generated by the eng2+-deficient mutant | Schizosaccharomyces pombe |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | Eng2 lacks a signal peptide for entry into the secretory pathway and therefore localizes intracellularly to the cytosol of the diploid cell | Schizosaccharomyces pombe | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | Schizosaccharomyces pombe | - |
? | - |
? | |
laminarin + H2O | Schizosaccharomyces pombe | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
several strains, overview | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | Eng2 is present during the mitotic cycle, and accumulates after meiosis II, expression of eng2+ is required for the efficient release of ascospores, expression of eng2+ is highly upregulated during the sporulation process, and the product is mainly involved in spore morphogenesis | Schizosaccharomyces pombe | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | - |
Schizosaccharomyces pombe | ? | - |
? | |
laminarin + H2O | - |
Schizosaccharomyces pombe | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucanase | - |
Schizosaccharomyces pombe |
Eng2 | - |
Schizosaccharomyces pombe |
More | the enzyme belongs to the glycosyl hydrolase family 81, GH81 | Schizosaccharomyces pombe |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Schizosaccharomyces pombe |
General Information | Comment | Organism |
---|---|---|
physiological function | When Schizosaccharomyces pombe diploid cells undergo meiosis, they differentiate into asci containing four haploid ascospores that are highly resistant to environmental stress. beta-Glucanase Eng2 and endo-alpha-1,3-glucanase Agn2 are required for ascus wall endolysis after sporulation in Schizosaccharomyces pombe | Schizosaccharomyces pombe |