Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and His affinity chromatography using ProBond nickel-chelating resin | Yarrowia lipolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
403 | - |
purified YlCrh2S, using laminarin as substrate, at 30°C | Yarrowia lipolytica |
3349 | - |
purified YlCrh1S, using laminarin as substrate, at 30°C | Yarrowia lipolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl-cellulose + H2O | carboxymethyl-cellulose is only hydrolyzed by YlCrh1Sp | Yarrowia lipolytica | ? | - |
? | |
laminarin + H2O | - |
Yarrowia lipolytica | D-glucose + ? | - |
? | |
lichenan + H2O | lichenan is only hydrolyzed by YlCrh1Sp | Yarrowia lipolytica | ? | - |
? | |
additional information | YlCrh2Sp does not hydrolyze carboxymethyl-cellulose, pustulan, and lichenan | Yarrowia lipolytica | ? | - |
? | |
pustulan + H2O | pustulan is only hydrolyzed by YlCrh1Sp | Yarrowia lipolytica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YlCrh1Sp | soluble enzyme form lacking the C-terminal consensus sequence for GPI anchoring and showing glycosidase activity on laminarin | Yarrowia lipolytica |
YlCrh2Sp | soluble enzyme form lacking the C-terminal consensus sequence for GPI anchoring and showing glycosidase activity on laminarin | Yarrowia lipolytica |