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Literature summary for 3.2.1.39 extracted from

  • Salazar, O.; Basso, C.; Barba, P.; Orellana, C.; Asenjo, J.A.
    Improvement of the lytic properties of a beta-1,3-glucanase by directed evolution (2006), Mol. Biotechnol., 33, 211-220.
    View publication on PubMed
Search for more literature for 3.2.1.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cellulosimicrobium cellulans

Protein Variants

Protein Variants Comment Organism
F160L increased activity Cellulosimicrobium cellulans
G145D increased activity Cellulosimicrobium cellulans
T149A/A344V increased activity Cellulosimicrobium cellulans
T149A/G145D/A344V increased activity Cellulosimicrobium cellulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 Laminarin mutant enzyme F160L, at 37°C Cellulosimicrobium cellulans
0.41
-
 Laminarin wild type enzyme, at 37°C Cellulosimicrobium cellulans
0.44
-
 Laminarin mutant enzyme T149A/A344V, at 37°C Cellulosimicrobium cellulans
0.46
-
 Laminarin mutant enzyme G145D, at 37°C Cellulosimicrobium cellulans
0.48
-
 Laminarin mutant enzyme T149A/G145D/A344V, at 37°C Cellulosimicrobium cellulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Cellulosimicrobium cellulans

Organism

Organism UniProt Comment Textmining
Cellulosimicrobium cellulans O68641 formerly Oerskovia xanthineolytica, strain LLG109
-

Purification (Commentary)

Purification (Comment) Organism
nickel metal affinity chromatography Cellulosimicrobium cellulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
49.1
-
 wild type enzyme, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans
70.3
-
 mutant enzyme F160L, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans
80.6
-
 mutant enzyme T149A/A344V, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans
96.2
-
 mutant enzyme G145D, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans
162.7
-
 mutant enzyme T149A/G145D/A344V, at 37°C, using laminarin as the substrate Cellulosimicrobium cellulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl-curdlan + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?
laminarin + H2O
-
 Cellulosimicrobium cellulans D-glucose + ?
-
 ?
lichenan + H2O
-
 Cellulosimicrobium cellulans D-glucose + ?
-
 ?
zymosan A + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-1,3-glucanase
-
 Cellulosimicrobium cellulans
BglII
-
 Cellulosimicrobium cellulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 Laminarin mutant enzyme T149A/A344V, at 37°C Cellulosimicrobium cellulans
35.9
-
 Laminarin wild type enzyme, at 37°C Cellulosimicrobium cellulans
51.5
-
 Laminarin mutant enzyme F160L, at 37°C Cellulosimicrobium cellulans
62.4
-
 Laminarin mutant enzyme G145D, at 37°C Cellulosimicrobium cellulans
72.8
-
 Laminarin mutant enzyme T149A/A344V, at 37°C Cellulosimicrobium cellulans
126.6
-
 Laminarin mutant enzyme T149A/G145D/A344V, at 37°C Cellulosimicrobium cellulans
138
-
 Laminarin mutant enzyme T149A/G145D/A344V, at 37°C Cellulosimicrobium cellulans