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Literature summary for 3.2.1.39 extracted from

  • Fuchs, K.P.; Zverlov, V.V.; Velikodvorskaya, G.A.; Lottspeich, F.; Schwarz, W.H.
    Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-beta-1,3-glucanase bound to the outer cell surface (2003), Microbiology, 149, 1021-1031.
    View publication on PubMed
Search for more literature for 3.2.1.39

Cloned(Commentary)

Cloned (Comment) Organism
-
 Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1-5 mM, strong Acetivibrio thermocellus
Fe2+ 1-5 mM, strong Acetivibrio thermocellus
Hg2+ 1-5 mM, strong Acetivibrio thermocellus
additional information not inhibitory: dithiothreitol, EDTA, ethanol up to 10%, SDS up to 20 mM, NaCl up to 50 mM Acetivibrio thermocellus
Zn2+ 1-5 mM, strong Acetivibrio thermocellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Acetivibrio thermocellus 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
 x * 148000, deduced from gene sequence, x * 125000, SDS-PAGE Acetivibrio thermocellus
148000
-
 x * 148000, deduced from gene sequence, x * 125000, SDS-PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus Q59328 strain DSM 1237, induction of enzyme by glucans containing 1-3-linkages
-
Acetivibrio thermocellus Q59328 induction of enzyme by glucans containing 1-3-linkages
-
Acetivibrio thermocellus F7 Q59328 induction of enzyme by glucans containing 1-3-linkages
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
340
-
 substrate laminarin, pH 6.0, 60°C Acetivibrio thermocellus
2680
-
 substrate barley beta-glucan, pH 6.0, 60°C Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
barley beta-glucan + H2O
-
 Acetivibrio thermocellus F7 ?
-
 ?
laminarin + H2O 13% of activity compared to barley beta-glucan Acetivibrio thermocellus ?
-
 ?
laminarin + H2O 13% of activity compared to barley beta-glucan Acetivibrio thermocellus F7 ?
-
 ?
lichenan + H2O 90% of activity compared to barley beta-glucan Acetivibrio thermocellus ?
-
 ?
lichenan + H2O 90% of activity compared to barley beta-glucan Acetivibrio thermocellus F7 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 148000, deduced from gene sequence, x * 125000, SDS-PAGE Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
Lic16A
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 2 h, stable Acetivibrio thermocellus
70
-
 10 min, 50% residual activity Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Acetivibrio thermocellus