Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibition at 1 mM | Porodisculus pendulus | |
Hg2+ | marked inhibition at 1 mM | Porodisculus pendulus | |
Pb2+ | inhibition at 1 mM | Porodisculus pendulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Laminarin | value in mg/ml | Porodisculus pendulus | |
additional information | - |
Laminarin | 3.04 | Porodisculus pendulus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Porodisculus pendulus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | 43000 | SDS-PAGE, gel filtration | Porodisculus pendulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pendulan + H2O | Porodisculus pendulus | acts in random fashion, reaction product with high molecular weight, major role in decreasing the molecular weight of pendulan during fermentation | reducing sugar + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porodisculus pendulus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, 5step chromatography | Porodisculus pendulus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Porodisculus pendulus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70.8 | - |
- |
Porodisculus pendulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O | end-product laminaribiose, no glucose release | Porodisculus pendulus | ? | - |
? | |
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O | laminaritetraose | Porodisculus pendulus | ? | - |
? | |
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O | end-products laminaritriose and laminaribiose, no glucose release | Porodisculus pendulus | ? | - |
? | |
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O | laminaripentaose | Porodisculus pendulus | ? | - |
? | |
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O | main product laminaritriose, small amounts laminaritriose and laminaribiose, no glucose release | Porodisculus pendulus | ? | - |
? | |
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O | laminarihexaose | Porodisculus pendulus | ? | - |
? | |
curdlan + H2O | - |
Porodisculus pendulus | ? | - |
? | |
laminarin + H2O | - |
Porodisculus pendulus | D-glucose + ? | - |
? | |
pendulan + H2O | acts in random fashion, reaction product with high molecular weight, major role in decreasing the molecular weight of pendulan during fermentation | Porodisculus pendulus | reducing sugar + ? | - |
? | |
schizophyllan + H2O | low activity | Porodisculus pendulus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000-43000, SDS-PAGE, gel filtration | Porodisculus pendulus |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,3-glucanase | - |
Porodisculus pendulus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Porodisculus pendulus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable below | Porodisculus pendulus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Porodisculus pendulus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.3 | stable within | Porodisculus pendulus |