General Stability | Organism |
---|---|
beta-glucanase IV highly unstable | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Laminarin | 25% inhibition above 2.5 mg/ml for beta-glucanase IIIB | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Laminarin | beta-glucanase I | Saccharomyces cerevisiae | |
0.04 | - |
Laminarin | beta-glucanase IIIB | Saccharomyces cerevisiae | |
0.21 | - |
Laminarin | beta-glucanase V | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | beta-glucanases I, IIIB, IV and V | Saccharomyces cerevisiae | 5618 | - |
intracellular | beta-glucanases I, IIIB and V | Saccharomyces cerevisiae | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69200 | - |
beta-glucanase V, native electrophoresis, gel filtration | Saccharomyces cerevisiae |
190500 | - |
beta-glucanase IIIB, native electrophoresis, gel filtration | Saccharomyces cerevisiae |
218000 | - |
beta-glucanase I, native electrophoresis, gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cell wall glucan + H2O | Saccharomyces cerevisiae | cell wall autolysis | reducing sugar + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, separation of four different beta-1,3-glucanases | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | four different isoforms, beta-glucanases I, IIIB, IV and V | Saccharomyces cerevisiae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
beta-glucanase IV | Saccharomyces cerevisiae |
1.12 | - |
beta-glucanase I | Saccharomyces cerevisiae |
1.21 | - |
beta-glucanase V | Saccharomyces cerevisiae |
9.62 | - |
beta-glucanase IIIB | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cell wall glucan + H2O | cell wall autolysis | Saccharomyces cerevisiae | reducing sugar + ? | - |
? | |
laminarin + H2O | different product patterns for glucanases I, IIIB, IV and V | Saccharomyces cerevisiae | D-glucose + ? | - |
? | |
periodate-oxidized laminarin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | for beta-glucanases I, IIIB and V | Saccharomyces cerevisiae |