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Literature summary for 3.2.1.39 extracted from

  • Usui, T.; Totani, K.; Totsuka, A.; Oguchi, M.
    Purification of endo-(1-3)-beta-D-glucanases lysing yeast cell walls from Rhizoctonia solani (1985), Biochim. Biophys. Acta, 840, 255-263.
No PubMed abstract available
Search for more literature for 3.2.1.39

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12500 20000 form L-4, SDS-PAGE, gel filtration Rhizoctonia solani
15000 23000 form L-2, SDS-PAGE, gel filtration Rhizoctonia solani
16000 22000 form L-1, SDS-PAGE, gel filtration Rhizoctonia solani

Organism

Organism UniProt Comment Textmining
Rhizoctonia solani
-
 differentiation into lytic and non-lytic endo-(1-3)-beta-glucanases against yeast cell walls, three lytic forms, L-1, L-2 and L-4, two non-lytic forms, N-1and N-2
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, three forms separated, L-1, L-2 and L-4, chromatography steps, affinity chromatography Rhizoctonia solani

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Rhizoctonia solani
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51
-
 form L-4 Rhizoctonia solani
99
-
 form L-1 Rhizoctonia solani
217
-
 form L-2 Rhizoctonia solani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pachyman + H2O insoluble pachyman, more affinity than on soluble, short-chain pachyman, forms L-1 and L-2 release predominantly pentaose, form L-4 yields a mixture from biose to heptaose Rhizoctonia solani ?
-
 ?
short-chain pachyman + H2O low affinity Rhizoctonia solani ?
-
 ?