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Literature summary for 3.2.1.39 extracted from

  • Prokop, A.; Rapp, P.; Wagner, F.
    Production, purification, and characterization of an extracellular endo-beta-1,3-glucanase from a monokaryon of Schizophyllum commune ATCC 38548 defective in exo-beta-1,3-glucanase formation (1994), Can. J. Microbiol., 40, 18-23.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ reduced activity to 41% and 50% at 1 and 5 mM Schizophyllum commune
Hg2+ complete inhibition at 1 mM Schizophyllum commune

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
Laminarin value in mg/ml Schizophyllum commune
additional information
-
Laminarin 0.28 Schizophyllum commune

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Schizophyllum commune
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
SDS-PAGE, gel filtration Schizophyllum commune

Organism

Organism UniProt Comment Textmining
Schizophyllum commune
-
defective in exo-beta-1,3-glucanase formation
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 3step chromatography Schizophyllum commune

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Schizophyllum commune
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
139.4
-
-
Schizophyllum commune

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1-3:1-4)-beta-D-glucan + H2O lichenan Schizophyllum commune ?
-
?
laminarin + H2O product distribution consistent of 20% laminaribiose, 30% laminaritriose and 50% higher oligosaccharides Schizophyllum commune D-glucose + ?
-
?
periodate-oxidized laminarin + H2O
-
Schizophyllum commune ?
-
?
schizophyllan + H2O from Schizophyllum commune, low activity Schizophyllum commune ?
-
?

Synonyms

Synonyms Comment Organism
endo-beta-1,3-glucanase
-
Schizophyllum commune

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Schizophyllum commune

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
fully active up to for 1 h Schizophyllum commune

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Schizophyllum commune

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7 stable within during 16 h Schizophyllum commune