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Literature summary for 3.2.1.38 extracted from

  • Colas, B.
    Some physicochemical and structural properties of two beta-fucosidases from Achatina balteata (1978), Biochim. Biophys. Acta, 527, 150-158.
    View publication on PubMed
Search for more literature for 3.2.1.38

General Stability

General Stability Organism
repeated freezing and thawing cycles don't affect fucosidase II, 30% loss of activity for beta-fucosidase I after 3 cycles Achatina balteata

Inhibitors

Inhibitors Comment Organism Structure
Urea activity of fucosidase I more rapidly lost than that of fucosidase II in 6 M urea Achatina balteata

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Achatina balteata
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000 120000 beta-fucosidase II, gel filtration, ultracentrifugation Achatina balteata
110000
-
 1 * 110000, beta-fucosidase II, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata
170000
-
 1 * 170000 + 1 * 190000, beta-fucosidase I, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata
190000
-
 1 * 170000 + 1 * 190000, beta-fucosidase I, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata
300000 360000 beta-fucosidase I, gel filtration, ultracentrifugation Achatina balteata

Organism

Organism UniProt Comment Textmining
Achatina balteata
-
 african snail
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains galactose, glucose, mannose, fucose, 15.5%, w/w, for beta-fucosidase I, 7.5%, w/w, for beta-fucosidase II, contains hexosamines, 6.1%, w/w, for beta-fucosidase I, 5.2%, w/w, for beta-fucosidase II, no sialic acid found Achatina balteata

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Achatina balteata

Renatured (Commentary)

Renatured (Comment) Organism
50% recovery for beta-fucosidase II, 25% recovery for beta-fucosidase I after treatment with 6 M urea Achatina balteata

Source Tissue

Source Tissue Comment Organism Textmining
digestive juice two forms, beta-fucosidase I and beta-fucosidase II Achatina balteata
-

Storage Stability

Storage Stability Organism
4°C, 50 mM sodium acetate, pH 5.4, several months Achatina balteata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-beta-D-fucoside + H2O
-
 Achatina balteata p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Achatina balteata p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucoside + H2O
-
 Achatina balteata p-nitrophenol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 170000 + 1 * 190000, beta-fucosidase I, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata
monomer 1 * 110000, beta-fucosidase II, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata

Synonyms

Synonyms Comment Organism
beta-fucosidase I
-
 Achatina balteata
beta-fucosidase II
-
 Achatina balteata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
 beta-fucosidase I, 60% loss of activity Achatina balteata
55
-
 beta-fucosidase II, 50% loss of activity Achatina balteata

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.5 beta-fucosidase II Achatina balteata
5.5 7 beta-fucosidase I Achatina balteata