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Literature summary for 3.2.1.38 extracted from
- Isolation, molecular properties and kinetic studies of a strict beta-fucosidase from Lactuca sativa latex (1988), Eur. J. Biochem., 175, 619-625.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | - |
Lactuca sativa |  |
| beta-D-fucose | competitive inhibitor | Lactuca sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
p-nitrophenyl-beta-D-fucoside | - |
Lactuca sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Lactuca sativa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
SDS-PAGE, gel filtration | Lactuca sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-fucoside + H2O | Lactuca sativa | implication in cell wall degradation | beta-D-fucose + 4-nitrophenol | the only products released from hypokotyl cell walls | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactuca sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 2step chromatography technique | Lactuca sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Lactuca sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 430 | - |
- |
Lactuca sativa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.01 M citrate pH 5.5, 0.02% sodium azide, several months | Lactuca sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-fucoside + H2O | implication in cell wall degradation | Lactuca sativa | beta-D-fucose + 4-nitrophenol | the only products released from hypokotyl cell walls | ? | |
| p-nitrophenyl-beta-D-fucoside + H2O | strict beta-D-fucosidase, no stereospecificity | Lactuca sativa | p-nitrophenol + beta-D-fucose | - |
? | |
| p-nitrophenyl-beta-L-fucoside + H2O | - |
Lactuca sativa | p-nitrophenol + beta-L-fucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 37000, SDS-PAGE, gel filtration | Lactuca sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
important inactivation at and above | Lactuca sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Lactuca sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | - |
Lactuca sativa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
rapid inactivation above | Lactuca sativa |
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