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Literature summary for 3.2.1.37 extracted from
- Highly efficient biotransformation of Astragaloside IV to cycloastragenol by sugar-stimulated beta-glucosidase and beta-xylosidase from Dictyoglomus thermophilum (2018), J. Microbiol. Biotechnol., 29, 1882-1893.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| methanol | activity is completely unaffected by low concentration of methanol and even enhanced by the concentration of methanol below 25% | Dictyoglomus thermophilum |  |
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | a method for bioconversion of 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol into cycloastragenol is optimized. A green and efficient biotransformation method is established for 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol using beta-glucosidase Dth3 and beta-xylosidase Xln-DT | Dictyoglomus thermophilum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously expressed in Escherichia coli BL21 (DE3) | Dictyoglomus thermophilum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Dictyoglomus thermophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyoglomus thermophilum | - |
- |
- |
| Dictyoglomus thermophilum DSM 3960 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dictyoglomus thermophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | activity is lower compared to 4-nitrophenyl-beta-D-xylopyranoside | Dictyoglomus thermophilum | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | activity is lower compared to 4-nitrophenyl-beta-D-xylopyranoside | Dictyoglomus thermophilum DSM 3960 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | activity is lower compared to 4-nitrophenyl-beta-D-xylopyranoside | Dictyoglomus thermophilum | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | activity is lower compared to 4-nitrophenyl-beta-D-xylopyranoside | Dictyoglomus thermophilum DSM 3960 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Dictyoglomus thermophilum | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Dictyoglomus thermophilum DSM 3960 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| additional information | no activity towards 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-rhamnopyranoside or 2-nitrophenyl beta-D-glucopyranoside | Dictyoglomus thermophilum | ? | - |
? | |
| additional information | no activity towards 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-rhamnopyranoside or 2-nitrophenyl beta-D-glucopyranoside | Dictyoglomus thermophilum DSM 3960 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Dictyoglomus thermophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-xylosidase | - |
Dictyoglomus thermophilum |
| Xln-DT | - |
Dictyoglomus thermophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Dictyoglomus thermophilum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | 70°C: about 50% of maximal activity, 90°C: about 65% of maximal activity | Dictyoglomus thermophilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
180 min, about 10% loss of activity | Dictyoglomus thermophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Dictyoglomus thermophilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | pH 4.5: about 65% of maximal activity, pH 6.0: about 40% of maximal activity | Dictyoglomus thermophilum |
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Release 2023.1 (January 2023)
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