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Literature summary for 3.2.1.37 extracted from

  • Zhang, S.; Xie, J.; Zhao, L.; Pei, J.; Su, E.; Xiao, W.; Wang, Z.
    Cloning, overexpression and characterization of a thermostable beta-xylosidase from Thermotoga petrophila and cooperated transformation of ginsenoside extract to ginsenoside 20(S)-Rg3 with a beta-glucosidase (2019), Bioorg. Chem., 85, 159-167 .
    View publication on PubMed
Search for more literature for 3.2.1.37

Activating Compound

Activating Compound Comment Organism Structure
ethanol activity is promoted to 116.6% by a concentration of methanol below 15% Thermotoga petrophila
methanol activity is promoted to 128.9% by a concentration of methanol below 15% Thermotoga petrophila

Application

Application Comment Organism
pharmacology cooperated transformation of ginsenoside extract to the pharmacologically active ginsenoside 20(S)-Rg3 with a beta-glucosidase. After transforming under optimal condition, the 20 g/l of ginsenoside extract is transformed into 6.28 g/l of Rg3 within 90 min, with a corresponding molar conversion of 95.0% and Rg3 productivity of 1793.49 mg/l/h, respectively Thermotoga petrophila

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermotoga petrophila

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 10% inhibition Thermotoga petrophila
DMSO
-
 Thermotoga petrophila
Hg2+ 1 mM, complete inhibition Thermotoga petrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.126
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.0, 90°C Thermotoga petrophila
2.684
-
 4-nitrophenyl alpha-L-arabinopyranoside pH 6.0, 90°C Thermotoga petrophila
6.165
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.0, 90°C Thermotoga petrophila

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, activity increases to 104% of control Thermotoga petrophila
K+ 1 mM, activity increases to 104% of control Thermotoga petrophila
Mg2+ 1 mM, activity increases to 105% of control Thermotoga petrophila
Sr2+ 1 mM, activity increases to 107% of control Thermotoga petrophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86700
-
 SDS-PAGE Thermotoga petrophila

Organism

Organism UniProt Comment Textmining
Thermotoga petrophila
-
-
-
Thermotoga petrophila DSM 13995
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga petrophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66.94
-
 substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 90°C Thermotoga petrophila
223.14
-
 substrate: 4-nitrophenyl alpha-L-arabinopyranoside, pH 6.0, 90°C Thermotoga petrophila
229.8
-
 substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 90°C Thermotoga petrophila
312.84
-
 substrate: 4-nitrophenyl alpha-L-arabinofuranoside, pH 6.0, 90°C Thermotoga petrophila
343.53
-
 substrate: beta-1,4-D-xylobiose, pH 6.0, 90°C Thermotoga petrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Thermotoga petrophila DSM 13995 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Thermotoga petrophila DSM 13995 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + beta-D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila DSM 13995 4-nitrophenol + beta-D-glucopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga petrophila DSM 13995 4-nitrophenol + beta-D-xylopyranose
-
 ?
beta-1,4-D-xylobiose + H2O
-
 Thermotoga petrophila beta-D-xylose
-
 ?
ginsenoside Rb2 + H2O
-
 Thermotoga petrophila ginsenoside Rd + alpha-L-arabinopyranose
-
 ?
ginsenoside Rc + H2O
-
 Thermotoga petrophila ginsenoside Rd + alpha-L-arabinofuranose
-
 ?
additional information no activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-L-rhamnopyranoside, 2-nitrophenyl beta-D-glucopyranoside Thermotoga petrophila ?
-
 ?
additional information no activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-L-rhamnopyranoside, 2-nitrophenyl beta-D-glucopyranoside Thermotoga petrophila DSM 13995 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 86700, SDS-PAGE Thermotoga petrophila

Synonyms

Synonyms Comment Organism
Tpexyl3
-
 Thermotoga petrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermotoga petrophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 100 activity is more than 50% of the maximum activity in the temperature range from 75°C to 100°C Thermotoga petrophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 residual activity is more than 85% of its original activity after being incubated at 70°C for 2 h Thermotoga petrophila
90
-
 residual activity is more than 50% of its original activity after being incubated at 90°C for 2 h Thermotoga petrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
982.321
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.0, 90°C Thermotoga petrophila
1000.73
-
 4-nitrophenyl alpha-L-arabinopyranoside pH 6.0, 90°C Thermotoga petrophila
2201.64
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.0, 90°C Thermotoga petrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Thermotoga petrophila

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 the enzyme retains over 60% of its optimum pH activity between pH 4.5 and pH 7.0 Thermotoga petrophila

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7.5 stable within a pH range of 4.0-7.5. The residual beta-xylosidase activity is more than 50% of the original purified enzyme activity after 1 h of incubation at 75 °C in the pH range from 4.5 to 7.5 at the absence of the substrate Thermotoga petrophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
373.25
-
 4-nitrophenyl alpha-L-arabinopyranoside pH 6.0, 90°C Thermotoga petrophila
457.9
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 6.0, 90°C Thermotoga petrophila
7808.73
-
 4-nitrophenyl beta-D-xylopyranoside pH 6.0, 90°C Thermotoga petrophila