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Literature summary for 3.2.1.37 extracted from
- Purification and characterisation of a thermostable beta-xylosidase from Aspergillus niger van Tieghem of potential application in lignocellulosic bioethanol production (2018), Appl. Biochem. Biotechnol., 186, 712-730 .
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | the relatively broad pH profile is favourable for industrial application as it offers potential flexibility in terms of process pH and is in line with the current pH range of lignocellulose enzymatic hydrolysis processes for bioethanol production | Aspergillus niger |
| industry | the relatively broad pH profile is favourable for industrial application as it offers potential flexibility in terms of process pH and is in line with the current pH range of lignocellulose enzymatic hydrolysis processes for bioethanol production | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arabinose | full activity is observed in the presence of up to 50 mM arabinose. Activity is reduced to 88%, 80% and 70% of maximum activity at 100 mM, 150 mM and 200 mM arabinose, respectively | Aspergillus niger |  |
| D-xylose | end-product inhibition | Aspergillus niger | |
| additional information | upon inclusion of glucose (10-200 mM) in the beta-xylosidase assay reaction mixture, the purified enzyme exhibits full activity relative to the control reaction without added glucose | Aspergillus niger | |
| SDS | 10 mM, 45% loss of activity | Aspergillus niger | |
| ZnSO4 | 10 mM, 50% loss of activity | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7526 | - |
4-nitrophenyl beta-D-xylopyranoside | 70°C, pH 5 | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger DSM 22593 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | treatment with PNGase F results in a decrease in molecular weight to approximately 85000 Da confirming that the purified enzyme is glycosylated | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | activity is 20% compared to activity with 4-nitrophenyl-beta-D-xylopyranoside | Aspergillus niger | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | activity is 20% compared to activity with 4-nitrophenyl-beta-D-xylopyranoside | Aspergillus niger DSM 22593 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | activity is 20% compared to activity with 4-nitrophenyl-beta-D-xylopyranoside | Aspergillus niger | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | activity is 20% compared to activity with 4-nitrophenyl-beta-D-xylopyranoside | Aspergillus niger DSM 22593 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus niger DSM 22593 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| beechwood xylan + H2O | the purified enzyme acts synergistically with Aspergillus niger endo-1,4-beta-xylanase in the hydrolysis of beechwood xylan | Aspergillus niger | ? | - |
? | |
| beechwood xylan + H2O | the purified enzyme acts synergistically with Aspergillus niger endo-1,4-beta-xylanase in the hydrolysis of beechwood xylan | Aspergillus niger DSM 22593 | ? | - |
? | |
| additional information | during hydrolysis of pretreated straw lignocellulose at 70 °C using a commercial lignocellulosic enzyme cocktail, inclusion of the purified enzyme results in a 19fold increase in the amount of xylose produced after 6 h. No activity is detected on 4-nitrophenyl beta-D-cellobioside | Aspergillus niger | ? | - |
? | |
| additional information | during hydrolysis of pretreated straw lignocellulose at 70 °C using a commercial lignocellulosic enzyme cocktail, inclusion of the purified enzyme results in a 19fold increase in the amount of xylose produced after 6 h. No activity is detected on 4-nitrophenyl beta-D-cellobioside | Aspergillus niger DSM 22593 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 120000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 75 | - |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 77 | over 50% of maximum activity is observed from 60°C to 77°C | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
24 h, enzyme retains full activity. 72 h, 9% loss of activity | Aspergillus niger |
| 65 | - |
24 h, enzyme retains full activity. 72 h, 13% loss of activity | Aspergillus niger |
| 70 | - |
18 h, 48% loss of activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.4 | 5.5 | over 69% of maximum activity is observed in the pH range 3.4 to 5.5 | Aspergillus niger |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.458 | - |
D-xylose | 70°C, pH 5 | Aspergillus niger | |
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