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Literature summary for 3.2.1.37 extracted from
- Expression and characterisation of a pH and salt tolerant, thermostable and xylose tolerant recombinant GH43 beta-xylosidase from Thermobifida halotolerans YIM 90462T for promoting hemicellulose degradation (2019), Antonie van Leeuwenhoek, 112, 339-350 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | the enzyme has potential for promoting hemicellulose degradation and other industrial applications | Thermobifida halotolerans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Thermobifida halotolerans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
p-nitrophenyl alpha-L-arabinoside | 55°C, pH 7.0 | Thermobifida halotolerans |  |
| 0.78 | - |
4-nitrophenyl beta-D-xyloside | 55°C, pH 7.0 | Thermobifida halotolerans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is not affected by K+, Mg2+, Fe2+, Fe3+, Ca2+, Co2+, Ba2+ | Thermobifida halotolerans | |
| NaCl | the enzyme displays high catalytic activity (60%) in presence of 5-35% NaCl (w/v) | Thermobifida halotolerans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61500 | - |
- |
Thermobifida halotolerans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida halotolerans | A0A399G7U0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermobifida halotolerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-xyloside + H2O | the enzyme displays high catalytic activity (60%) in presence of 5-35% NaCl (w/v) or 1-20% ionic liquid (w/v) or 1-50 mM xylose | Thermobifida halotolerans | 4-nitrophenol + beta-D-xylose | - |
? | |
| additional information | no activity is found for beechwood xylan and cellobiose | Thermobifida halotolerans | ? | - |
? | |
| p-nitrophenyl alpha-L-arabinoside + H2O | about 6% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Thermobifida halotolerans | 4-nitrophenol + alpha-L-arabinose | - |
? | |
| xylobiose + H2O | 25% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Thermobifida halotolerans | 2 beta-D-xylose | - |
? | |
| xylotetraose + H2O | 21% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Thermobifida halotolerans | xylotriose + beta-D-xylose | - |
? | |
| xylotriose + 2 H2O | about 12.1% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Thermobifida halotolerans | 3 D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 61500 | Thermobifida halotolerans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Thxyl43A | - |
Thermobifida halotolerans |
| xyl43A | - |
Thermobifida halotolerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Thermobifida halotolerans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 60 | 15°C: 27 % of maximal activity, 25°C: 40% of maximal activity, 60°C: 60% of maximal activity | Thermobifida halotolerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the enzyme retains more than 80% activity after incubation for 66 h | Thermobifida halotolerans |
| 50 | - |
the enzyme retains more than 80% activity after incubation for 66 h | Thermobifida halotolerans |
| 55 | - |
t1/2: 29.7 h. The enzyme retains 47% of its activity after 30 h incubation at 55°C, the enzyme retains 90% of its activity after 24 h incubation at 55°C | Thermobifida halotolerans |
| 60 | - |
t1/2: 4.8 h | Thermobifida halotolerans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
p-nitrophenyl alpha-L-arabinoside | 55°C, pH 7.0 | Thermobifida halotolerans | |
| 50 | - |
4-nitrophenyl beta-D-xyloside | 55°C, pH 7.0 | Thermobifida halotolerans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermobifida halotolerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10.6 | the enzyme is notably active at pH 4-6 and pH 9-10. At pH 8.0 about 65% and 90% relative activity is detected in citrate buffer and borate buffer respectively. At pH 8.6 and 9.0 less than 65% relative activity is observed in borate buffer, while more than 85% is found in Gly-NaOH buffer. More than 60% relative activity is noted at pH 10.6 | Thermobifida halotolerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 12 | the enzyme retains more than 80% residual activity after incubation at pH range of 4.0 to 12.0 for 24 h | Thermobifida halotolerans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
p-nitrophenyl alpha-L-arabinoside | 55°C, pH 7.0 | Thermobifida halotolerans | |
| 64.1 | - |
4-nitrophenyl beta-D-xyloside | 55°C, pH 7.0 | Thermobifida halotolerans | |
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