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Literature summary for 3.2.1.37 extracted from

  • Bao, L.; Huang, Q.; Chang, L.; Sun, Q.; Zhou, J.; Lu, H.
    Cloning and characterization of two beta-glucosidase/xylosidase enzymes from yak rumen metagenome (2012), Appl. Biochem. Biotechnol., 166, 72-86.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ethanol 5% (v/v), 316.9% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
methanol 5% (v/v), 167% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and purified uncultured rumen bacterium

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular docking studies for the enzyme with the substrates xylobiose and 4-nitrophenyl-beta-D-xylopyranoside. The essential catalytic residues of RuBG3A are D297 and E499. Besides D113, K218, H219, and R185 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters uncultured rumen bacterium
molecular docking studies for the enzyme with the substrates xylobiose and 4-nitrophenyl-beta-D-xylopyranoside. The essential catalytic residues of RuBG3B are D272 and E481. Besides D88, K193, H194, and R160 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters uncultured rumen bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1317
-
4-nitrophenyl beta-D-xylopyranoside pH 4.6, 42°C uncultured rumen bacterium
65
-
4-nitrophenyl beta-D-xylopyranoside pH 4.6, 40°C uncultured rumen bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
CaCl2 5 mM, 99% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
CoCl2 5 mM, 89.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
CoCl2 5 mM, 91.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
CuCl2 5 mM, 87.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
CuCl2 5 mM, 96.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
D-fructose 5 mM, 76.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
D-fructose 5 mM, 86.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
D-glucose 5 mM, 4.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
D-glucose 5 mM, 54.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
D-xylose 5 mM, 81.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
D-xylose 5 mM, 97.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
MgCl2 5 mM, 87.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
MgCl2 5 mM, 94.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
MnCl2 5 mM, 93.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
MnCl2 5 mM, 98.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
NiCl2 5 mM, 96.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
NiCl2 5 mM, 98.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
ZnSO4 5 mM, 101.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
ZnSO4 5 mM, 99.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 5% (v/v), 112.7% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
Ethanol 5% (v/v), 316.9% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
Methanol 5% (v/v), 167% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
Methanol 5% (v/v), 94.3% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium

Organism

Organism UniProt Comment Textmining
uncultured rumen bacterium G3BJX3 Rubg3A, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
-
uncultured rumen bacterium G3BJX4 Rubg3B, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme uncultured rumen bacterium

Source Tissue

Source Tissue Comment Organism Textmining
additional information isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens uncultured rumen bacterium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5
-
4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
12.1
-
4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O 15.7% relative activity compared to the beta-glucosidase activity with 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium 4-nitrophenol + D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O 21.1% relative activity compared to the beta-glucosidase activity with 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium 4-nitrophenol + D-xylose
-
?
additional information RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3A with an activity ratio of 10:1 (xylosidase/xylanase) increases the liberation to 218% of that in the presence of XynR8 alone uncultured rumen bacterium ?
-
?
additional information RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3B with an activity ratio of 5:1 (xylosidase/xylanase) increases the liberation to 169% of that in the presence of XynR8 alone. RuBG3B shows no synergism with xylanase XynR8 at the optimal pH 7.5, but at pH 5.5, indicating that RuBG3B may have different pH preferences between aryl xylosidic substrate 4-nitrophenyl-beta-D-xylopyranoside and xylooligisaccharide xylan uncultured rumen bacterium ?
-
?
xylobiose + H2O
-
uncultured rumen bacterium 2 D-xylose
-
?

Synonyms

Synonyms Comment Organism
beta-glucosidase/xylosidase bifunctional, belongs to to the glycoside hydrolase 3 familiy, GH3 uncultured rumen bacterium
beta-xylosidase
-
uncultured rumen bacterium
Rubg3A gene name, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74 uncultured rumen bacterium
Rubg3B gene name, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74 uncultured rumen bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
optimal, with 4-nitrophenyl-beta-D-xylopyranoside as substrate uncultured rumen bacterium
40
-
assay at uncultured rumen bacterium
40
-
optimal, with 4-nitrophenyl-beta-D-xylopyranoside uncultured rumen bacterium
42
-
assay at uncultured rumen bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
4-nitrophenyl beta-D-xylopyranoside pH 4.6, 42°C uncultured rumen bacterium
135
-
4-nitrophenyl beta-D-xylopyranoside pH 4.6, 40°C uncultured rumen bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
assay at uncultured rumen bacterium
5.5
-
optimal, with 4-nitrophenyl beta-D-xylopyranoside as substrate uncultured rumen bacterium
7.5
-
optimal, with 4-nitrophenyl beta-D-xylopyranoside as substrate uncultured rumen bacterium

General Information

General Information Comment Organism
physiological function RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3A with an activity ratio of 10:1 (xylosidase/xylanase) increases the liberation to 218% of that in the presence of XynR8 alone uncultured rumen bacterium
physiological function RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3B with an activity ratio of 5:1 (xylosidase/xylanase) increases the liberation to 169% of that in the presence of XynR8 alone. RuBG3B shows no synergism with xylanase XynR8 at the optimal pH 7.5, but at pH 5.5, indicating that RuBG3B may have different pH preferences between aryl xylosidic substrate 4-nitrophenyl-beta-D-xylopyranoside and xylooligisaccharide xylan uncultured rumen bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2084
-
4-nitrophenyl beta-D-xylopyranoside pH 4.6, 40°C uncultured rumen bacterium
54080
-
4-nitrophenyl beta-D-xylopyranoside pH 4.6, 42°C uncultured rumen bacterium