Application | Comment | Organism |
---|---|---|
synthesis | immobilization of enzyme on chitosan as best support material gives immobilization and activity yields of 94% and 87%, respectively, of initial activity, and also provides the highest stability, retaining 94% of its initial activity even after being recycled 25times. Maximal activity of immobilized enzyme is achieved at pH 8.0 and 53°C, whereas that for the free enzyme is obtained at pH 7.0 and 50°C. The immobilized enzyme is more thermostable than the free beta-xylosidase. Km values of the free enzyme increases from 2.37 mM to 3.42 mM at the immobilized state. Immobilized enzyme catalyzes the reverse hydrolysis reaction, forming xylooligosaccharides in the presence of a high concentration of xylose. Co-immobilized of beta-xylosidase and xylanase on chitosan leads to a continuous hydrolysis of 3% oat spelt xylan at 50°C and better hydrolysis yields and higher amount of xylose are obtained | Thermomyces dupontii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 70% residual activity of native enzyme, 96% of immobilized enzyme | Thermomyces dupontii | |
EDTA | 5 mM, 55% residual activity of native enzyme, 63% of immobilized enzyme | Thermomyces dupontii | |
Fe2+ | 5 mM, 20% residual activity of native enzyme, 42% of immobilized enzyme | Thermomyces dupontii | |
Hg2+ | 5 mM, 49% residual activity of native enzyme, 64% of immobilized enzyme | Thermomyces dupontii | |
SDS | 5 mM, 44% residual activity of native enzyme, 65% of immobilized enzyme | Thermomyces dupontii | |
Zn2+ | 5 mM, 34% residual activity of native enzyme, 56% of immobilized enzyme | Thermomyces dupontii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.37 | - |
4-nitrophenyl-beta-D-xylopyranoside | native enzyme, pH 7.0, 50°C | Thermomyces dupontii | |
3.42 | - |
4-nitrophenyl-beta-D-xylopyranoside | immobilized enzyme, pH 7.0, 50°C | Thermomyces dupontii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 45% stimulation of native enzyme, 20% of immobilized enzyme | Thermomyces dupontii | |
Co2+ | 5 mM, 22% stimulation of native enzyme, 11% of immobilized enzyme | Thermomyces dupontii | |
Mn2+ | 5 mM, 30% stimulation of native enzyme, 28% of immobilized enzyme | Thermomyces dupontii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces dupontii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 D-xylose | - |
Thermomyces dupontii | xylobiose + H2O | and higher xylooligosaccharides, immobilized enzyme in presence of high concentration of xylose | r | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermomyces dupontii | 4-nitrophenol + beta-D-xylose | - |
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