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Literature summary for 3.2.1.37 extracted from
- Characterization of a highly efficient heterodimeric xylosidase from Humicola insolens (2009), Enzyme Microb. Technol., 45, 436-442.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | 10-20% stimulation | Humicola insolens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 90% inhibition | Humicola insolens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.74 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.6, 40°C | Humicola insolens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10-20% stimulation | Humicola insolens | |
| Mg2+ | 10-20% stimulation | Humicola insolens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
1 * 68000, catalytic subunit, 1 * 17000, carbohydrate binding subunit, SDS-PAGE | Humicola insolens |
| 68000 | - |
1 * 68000, catalytic subunit, 1 * 17000, carbohydrate binding subunit, SDS-PAGE | Humicola insolens |
| 84000 | - |
analytical ultracentrifugation | Humicola insolens |
| 92200 | - |
sedimentation velocity analysis | Humicola insolens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Humicola insolens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Humicola insolens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Humicola insolens | 4-nitrophenol + D-xylopyranose | - |
? | |
| additional information | no substrate: 4-nitrophenyl-alpha-L-arabinofuranoside | Humicola insolens | ? | - |
? | |
| xylobiose + H2O | - |
Humicola insolens | 2 D-xylose | - |
? | |
| xylotriose + H2O | - |
Humicola insolens | 3 D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 68000, catalytic subunit, 1 * 17000, carbohydrate binding subunit, SDS-PAGE | Humicola insolens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Humicola insolens |
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Release 2023.1 (January 2023)
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